Contamination of aquatic ecosystems by heavy metals is considered one of the most major threats of the aquatic life. So, the present work aims to investigate the accumulation and tolerance of Ceratophyllum demersum plant to different concentrations of Pb+2, Al+3 and Cd+2 during four treatment periods for 16 days. Results indicated that the highest tolerance percentage of C. demersum was (96.26 %) of Pb at 10 mg L-1 after 4 days; whereas the highest tolerance percentage values were (95.89 % and 80.84 %) at 3 mgL-1 of Al and at 0.1 mg L-1 of Cd after 8 days, respectively. C. demersum accumulated the highest amount of Pb (17029.19 μg g-1) at 50 mg L-1 after 12 days, followed by Al amount which was (2893.04 μg g-1) at 9 mg L-1 after 8 days and the highest accumulation value of Cd was (521.28 μg g-1) at 0.5 mg L-1 after 4 days. Metal accumulation in C. demersum showed at a descending order as follow; Pb >Al >Cd and it was significantly increased with increasing the concentration and treatment period. In addition, results showed that the maximum BCF value of Cd in C. demersum at low concentration was (1295.37) after 12 days of the treatment. Therefore, C. demersum is considered a hyperaccumulator species for cadmium. © 2018, Egyptian Society for the Development of Fisheries and Human Health. All rights reserved.