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Biochemistry and Molecular Biology Department

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Attain significant research heights and recognition as leader Molecular biology, genetic engineering, biotechnology and Nanobiotechnology research center. Shaping this research into innovative applicable tools for generation of knowledge, processes and products for benefit of the society and nation.

The mission of our department is to maximize the benefits of bioscience and biotechnology to our institute, environment and nation by being an excellent quality, comprehensive, multidisciplinary research, training and services center, that coordinates, disseminates and advances biotechnology and its applications for the benefit of society and human health. This will be through continuous learning to enhance the knowledge and skills that are of value to our staff.

Email: [email protected]، [email protected]



 

 

Former Heads 

Staff members

Biochemistry Department has a long history in the field of molecular biology since 1981, when Prof.  Dr. Mohamed Ali Saber started to establish the molecular biology trends and facilities in biochemistry lab.

Different trends of research have been adopted as follows:

1- Schistosomiasis research:

    •  Biochemical and molecular level: Including host and parasite metabolism, molecular events study, recombinant and DNA vaccine development and genome characterization.

2- Liver research:

    •  Including molecular studies using in situ hybridization techniques and studies in liver cells culturing and transplantation.

3-Molecular diagnosis and characterization of infectious pathogens:

    •  Molecular characterization of multi drug resistant bacteria and development of real time PCR assays and kits (Including HCV, HBV, HPV, CMV, H. Pylori etc.)

4- Response to treatment:

    •  interferon treatment studies for hepatitis C Virus & other pathogens

5- R& D for production of recombinant biopharmaceuticals 

    •  Development of lab scale expression and production for selected therapeutic proteins (interferon alpha 2b, interferon gamma, IL12, growth hormone and granulocyte colony stimulating factor).
    •  Optimization of R& D for pilot scale production of recombinant proteins.

6- Nanotechnology application in diagnosis and drug delivery

    •   Designing, bio conjugation and characterization of Nano- and micro-carrier systems (colloidal nanoparticles, polyelectrolyte multilayer capsules, etc) for controlled delivery of therapeutic drugs and genetic materials into targeted cells.

The utilization of nanoparticles to develop novel diagnostic tests for detection of biomarkers of disease and pathogens and other bio-applications.

1. Certain biochemical, pathophysiological and molecular studies in schistosomiasis have been performed on both parasite and host-liver.

2. Development of specific in-situ hybridization techniques, for detection of specific DNA/RNA sequences in liver tissues.

3. Molecular diagnostic and epidemiological studies on hepatitis viruses (B, C and G), Sin virus, papilloma virus, CMV, EBV.

4. Molecular diagnostic and epidemiological studies on pathogens (Salmonella, Helicobacter pylori and Listeria etc.).

5. Molecular Studies on response of HCV infected patients to treatment using recombinant interferon alfa.

6. Biochemical, Epidemiological and Molecular studies of HCV in Egypt.

7. Isolation and molecular identification of four schistosome genes (aldolase, fimbrin, Sm21.7, glucose phosphate isomerase,) (www.ncbi.nlm.nih.gov). These genes have been evaluated as schistosoma vaccine candidates as proteins and as DNA vaccines.

8. Construction of Schistosoma mansoni expression libraries of different life cycle stages which have been used by several countries (distributed internationally through the “Schistosome Geneome Project Network”, www.nhm.ac.uk/hosted_sites/schisto).

9. Isolation and identification of Schistosoma mansoni ESTS through contribution in Schistosome genome project (Saber MA, www.ncbi.nlm.nih.gov). More than 1650 Scistosoma ESTs are deposited in the GenBank of the NCBI.

10. Hepatocytes isolation and transplantation into experimental animals, either syngenic or xenogenic transplantation. Hepatocytes were immuno-modulated or immuno-isolated and transplanted intrasplenic or intraperitoneal, after induction of acute hepatic failure in transplanted animals.

11. Identification of new mutations in the determinant region of the hepatitis B virus genome isolated from Egyptian patients negative for HBsAb.

12. In collaboration with VACSERA, a construction of recombinant DNA vaccine for the Egyptian strain of foot and mouth disease virus has been developed.

13. Molecular studies on praziquantel resistant Schistosoma mansoni strains.

14. Lab scale production of human interferon alfa 2b, Interferon gamma, growth hormone, IL2 and granulocyte colony stimulating factor.

15. Bacteriological and molecular study of multi drug resistant Mycobacterium tuberculosis in Egypt.

16. Study the role of bacterial DNA detection in diagnosis of SBP in patients with cirrhosis and culture negative ascites.

17. Development of a rapid detection method of methiciline resistant Staphylococcus aurous SPP bacteraemia among ICU patients.

18. Study the effect of whey protein on hepatitis C virus patients.

19. Development and evaluation of S. mansoni DNA vaccines using different routs of injection.

20. R&D for limited pilot scale production process for IFN alpha 2b including fermentation, protein folding and purification and activity assay.

21. Study the genetic factors controlling HCV response to IFN therapy for both the virus and host.

22. Development of Quantitative Real time PCR assay for Detection of Hepatitis B Virus.

23. Development of protocols for Nano and Micro capsules synthesis for drug delivery.

24. Development of Nanotechnology-based drug delivery vehicle for treatment of multi-drug-resistant tuberculosis.

25. Development of a Novel Assay for Direct detection of Unamplified Hepatitis C Virus RNA Using Gold Nanoparticles.

26. Development of Gold Nanoparticle-Based Colorimetric Assay for the Direct Detection of liver Cancerous Cells and biomarkers.

27. Synthesis of some nanoparticles (gold, Silver, etc.)

28. Quality control lab for the MOH national survey in 2008 & 2015 of HCV, HBV in Egypt.

Gene isolation, cloning and characterization:
Including isolation of genetic materials, PCR, DNA sequencing, DNA labeling and modification, Northern blot and Southern blot, In situ hybridization, construction of genomic and cDNA expression libraries, ESTs development and gene Cloning.

Recombinant protein expression:
 in different E. coli systems, shake flask culture and fermentation, protein folding, one and 2 D protein electrophoresis, protein purification using chromatography and quantification, HPLC analysis, preparative isoelectric focusing and activity assays.

Immunological characterization:
Development of specific polyvalent antibodies, sera treatment, IgG purification, expression libraries immuno-screening, Western blot, dot blot, ELISA, immunohistochemistry and immuno-affinity chromatography.

–In-vitro and In-vivo culturing of hepatocytes:
-Hepatocytes culture, immuno-modulation and transplantation (in spleen and peritoneum).
-MDBK and VERO Cells, and vesicular stomatitis virus (VSV) for antiviral assays.

Bioinformatics:
Retrieve and analysis of DNA and protein sequences, data bases search, primer design, notification of unknown sequences, design of gene models for recombinant protein expression in different host cells.

Molecular diagnosis:
– PCR and real time PCR for detection and quantification of infectious agents (see routine services).
– In situ hybridization, reverse hybridization, array hybridization.

Designing of nano- and micro-carrier systems for Cargo delivery:
Synthesis and characterization of nano and micro capsules for controlled delivery of therapeutic drugs and genetic materials into targeted cells (synthesis of capsules, coating, functionalization, cargo loading, encapsulation efficiency, cargo release, cellular uptake, Investigation of the cytotoxic effects the capsules on cells, plasma stability, etc ).

Utilization of nanoparticles to develop novel diagnostic tests: 
For detection of biomarkers of diseases and pathogens (NPs synthesis, characterization and Functionalization, NPs conjugation with oligonucleotide probes).

In addition to the highly experienced staff, Biochemistry and Molecular Biology Department at Theodor Bilharz Research Institute is a well equipped molecular biology and genetic engineering laboratory with well established domains and equipment for different specific fields such as, genetic material isolation, PCR, cloning, cell culturing, protein expression, protein purification, immuno-characterization (ELISA, Western, etc.) and bioinformatics. In 2005 we have established “Biotechnology and Genetic Engineering Unit” for the purpose of R & D in pilot scale production of important proteins especially biopharmaceuticals. This unit has fermentation facilities, protein purification and refolding facilities and tissue culture facilities for biological evaluation of activity of the produced recombinant proteins.NanoBiotechnology unit has been established in 2016 in to promote and support the development of new systems for diagnostics and disease treatment through the research in one of the most promising fields within the new technological advances: the nanoscience.

Major instruments are:

  1.  Fermentor (BioFlo110, New Brunswick, USA). For bacterial cells and mammalian cells.
  2.  French Pressure haemogenizer (Nero).
  3.  Liquid chromatographic system (AKTA Purifier, GE Health Care).
  4.  Ultra speed and cooling centrifuges.
  5. DNA sequencer.
  6.  DNA extraction system and speed vacuum.
  7. Real time PCR and conventional PCR instruments (gradient, in situ).
  8.  Nano Drop spectrophotometer instrument.
  9. CO2 incubators.
  10. Laminar flow cabinets.
  11. Agarose gel and PAGE electrophoresis systemsG
  12. Gel documentation systems
  13. One dimension and 2 dimension protein electrophoresis and transfer systems.
  14. Isoelectric focusing systems.
  15.  FPLC chromatographic systems.
  16. Inverted phase and fluorescence and optical microscopes.
  17.  Spectrophotometers.
  18. Deep freezers (-20 and -70) and autoclaving systems.
  19. Environmental shaker.
  20.  ELISA reader.


Biochemistry and Molecular biology Department at TBRI had kept steady record for holding local, national and international training workshops (hands on) in different aspects of molecular biology, biotechnology and Nanobiotecnology as follows:

  1.  Biochemistry and Molecular Biology Department is considered by WHO as reference training center for African and Arabian countries concerning molecular diagnosis of infectious diseases
  2.  Training courses in principles of molecular biology.
  3.  Organizing workshops on molecular forensic diagnosis.
  4.   Workshops on real time PCR.
  5.   Workshops on Nanotechniques.
  6.   Workshops in recombinant protein expression in E. Coli systems.
  7.   In collaboration with universites, selected graduation research projects have been conducted at Biochemistry and Molecular biology Dept., TBRI.

Customized training workshops can be organized in many other different fields for groups.


For more than 20 years, Biochemistry and Molecular Biology Department has introduced wide scope of services for society, scientific community and organizations. Three major services have been carried out:

  1. Molecular diagnosis of diseases and Pathogens for in and out patients (see Routine out & in patients services),
  2. Workshops and training (see Training facilities).
  3. scientific services
      •   Isolation of genetic material.
      •   Design of PCR primers and PCR amplification.
      •   DNA and protein electrophoresis.
      •   Gene isolation, cloning, protein expression and protein purification.
      •   Investigation of the cytotoxicity of nanoparticles, drugs and compounds.


The Molecular Diagnostic Unit in Biochemistry and molecular Biology Department serve the molecular diagnosis of hepatitis viruses and other viruses, in addition to pathogenic bacteria, using polymerase chain Reaction (PCR), real time PCR, reverse hybridization and other techniques for qualitative and quantitative assay for in and out patients. The following assays are available.

1-  HCV-RNA (Quantitative)
2-  HCV-RNA (Qualitative)
3-  HCV-RNAtriple test
4-  IL28 gene variants.
5-   IL4 variants.
6-   HCV-Genotyping.
7-   HBV-DNA by PCR (Quantitative)
8-   HBV-DNA by PCR (Qualitative)
9-   HGV-RNA by PCR (Qualitaive)
10-  CMV-DNA by PCR (Qualitative)
11- TB-DNA by PCR (Qualitative)
12- H. pylori by PCR
13- Cystic Fibrosis by PCR
14- Philadelphia Chromosome by PCR (Qualitative) .
15- HPV & Genotyping by qRT-PCR

Internal projects:

    1. TBRI: ID 80 ش Development of real time PCR assay for detection of hepatitis B virus. PI: Professor Dr Mohamed Abbas Shemis

    2. TBRI ID 16ك: The role of microRNA as a diagnostic biomarker in bladder cancer Egyptian patients.(2016) PI: Dr. Samah Mamdouh.

    3. TBRI: Microsatellite instability in hepatocellular carcinoma associated with hepatitis C virus infection in Egyptian patients.(2015) PI: Dr. Samah Mamdouh.

    4. TBRI ID 16ك (2014): Biochemical studies on purified recombinant interferon gamma produced in E. coli

    5. TBRI: ID 1 (innovative research). (2008-2012) Optimization of expression and activity of interferon alfa-2b produced in E. coli. PI: Professor Dr Ehab El-Dabaa

External projects:

    1. Academy of Scientific Research and Technology (2020): Development and Validation of a National Diagnostic Assay for Rapid Detection of COVID19. PI: Professor Dr Mohamed Abbas Shemis.

    2. Academy of Scientific Research and Technology (2018-2020): “Scaling-up, Production & commercialization of Real Time-PCR kit for HBV diagnosis”. . PI: Professor Dr Mohamed Abbas Shemis.

    3. STDF, ID: 25901 (2018): “Long Non-Coding RNA: An Innovative Therapeutic Tool for Hepatocellular Carcinoma in vitro and in vivo”. PI: Prof. Dr. Eman El Ahwany.

    4. STDF, ID: 27797 (2018): “Polymer Nanoparticle Mediated Delivery of MicroRNA: A Novel Potential Therapeutic Tool for Hepatocellular Carcinoma in an Experimental Model”. PI: Professor Dr Eman El Ahwany.

    5. STDF – DAAD (2017): “Assessment of potential synergistic or antagonistic toxicity mechanisms during co-exposition of in vitro models towards cerium dioxide nanoparticles and environmental chemicals/pharmaceuticals“. PI: Professor Dr Mohamed Abbas Shemis.

    6. AECID, Spain: Development of Gold Nanoparticle-Based Colorimetric Assay for the Direct Detection of liver Cancerous Cells and biomarkers”. PI: Professor Dr Mohamed Abbas Shemis.

    7. Ministry of Scientific Research `Egypt` & National Research Foundation `South Africa,` ID17-2-12: ’Nanotechnology-based drug delivery for treatment of multi-drug-resistant tuberculosis. PI: Professor Dr Mohamed Abbas Shemis.

    8. STDF-DAAD: Multilayer Polyelectrolyte Microcapsules: a Novel Tool for Controlled Delivery of Interferon-alpha in Chronic HCV Infected Patients. PI: Professor Dr Mohamed Abbas Shemis.

    9. ASRT Strategy ID (2014-2017). Scalling up of production of recombinant human interferon Gamma in prokaryotic system. PI: Professor Dr Mohamed Ali Saber

    10. ASRT (2014-2016): Development of a Novel Assay for Direct Quantification of Unamplified Hepatitis C Virus RNA Using Gold Nanoparticles and Catalytic Signal Amplification’’. PI: Professor Dr Mohamed Abbas Shemis

    11. STDF – STF ID 6080. (2013-2016) ”Biochemical and Biophysical studies on recombinant human growth hormone expressed in a prokaryotic system”. Assistant Researcher Sami Mohamed Nasr

    12. Ministry of scientific research &NRF `South Africa: ID17 (2012-) :”Nanotechnology-based drug delivery for treatment of multi-drug-resistant tuberculosis’’. PI: Professor Dr Mohamed Abbas Shemis

    13. STDF ID 1454. Development of production and pegylation of interferon alph Principle investigator: Professor Dr Ehab El-Dabaa. 2010 -2014.

    14. STDF Egypt ID 1763 (2010-2014) Impact of interferon alpha-2 alleles and anti-interferon alpha-2 antibodies in HCV infected patients as predictive factors for response to interferon therapy. PI: Professor Dr Mohamed Ali Saber.


2020

    1. Lesego L. Tshweuab, Mohamed A. Shemis, Aya Abdelghanyc, Abdullah Goudac, Lynne A. Pilcher, Nicole R. S. Sibuyi, Mervin Meyer, Admire Dube and Mohammed O. Balogun (2020). Synthesis, physicochemical characterization, toxicity and efficacy of a PEG conjugate and a hybrid PEG conjugate nanoparticle formulation of the antibiotic moxifloxacin. RSC Adv., 2020, 10, 19770.

    2. Okasha, H. (2020). Interferon and p 53 Tumor Suppressor Marker in Hepatocellular Carcinoma. International Journal of Pharmaceutical Research, 12(02).

    3. Morsi, E. A., Ahmed, H. O., Abdel-Hady, H., El-Sayed, M., & Shemis, M. A. (2020). GC-analysis, Antioxidant, Anti-inflammatory, and Anticancer Activities of some extracts and fractions of Linum usitatissimum. Current Bioactive Compounds, 16.

    4. Hend Okasha, Safia Samir (2020): Synthesis and molecular cloning of antimicrobial peptide chromogranin A N-46 gene using conventional PCR. Gene Reports 18 (2020) 100571.

2019

    1. Khaled Mohamed Farid El Sehrawy, Mohamed Ali El-Dessouky, Sanaa Osama Abdallah, Moataz Hassan, Hanan Omar Hassanein, Mohamed Abbas Shemis, Mohamed Mahmoud Shaheen, Mohamed Ali Saber (2019). Correlation of Response of Pegylated Interferon and Ribavirin to Mutations in the Interferon Sensitivity-Determining Region and Internal Ribosome Entry Sites of HCV Genome Isolated from Egyptian Patients. Life Science Journal. 16 (1).

    2. AR Mashaal, M Abd El-Hameed, AA El Ray, MA Shemis, M Seyam (2019). Detection Of Occult Hepatitis C Virus Infection In Egyptian Patients Who Achieved A Sustained Virologic Response To Direct-Acting Antiviral Agents. Hepatology. 70, 921A-921A.

    3. Manal Diab, Dalia Salem, Ahmed El-Shenawy, Amira El-Far, Aya Abdelghany, Alaa Reda Awad, Inas El Defrawy, Mohamed Shemis (2019). Detection of high level aminoglycoside resistance genes among clinical isolates of Enterococcus species . Egyptian Journal of Medical Human Genetics. 20 (1), 28.

    4. Hend Okasha, Marwa Hassan, Tarek Aboushousha, & Safia Samir. (2019). Effect of Interferon-Beta (IFN-β) on tumor suppressor and apoptotic markers in hepatocellular carcinoma cell line. International Journal of Research in Pharmaceutical Sciences, 10(4), 2936–2943.

    5. Marwa T. A Abdel-Wareth, Ali M. El-Hagrassi, Mohamed S. Abdel-Aziz, Sami M. Nasr and Mosad A. Ghareeb (2019) Biological activities of endozoic fungi isolated from Biomphalaria alexandrina snails maintained in different environmental conditions. International Journal of Environmental Studies , 76:5, P 780:799.

    6. – Heba A. Hady, Eman A. El Wakil, Sami M. Nasr (2019). Characterization and Evaluation of Antimicrobial and Cytotoxic Activities of Glycine max Methanol Extract. International Journal of Pharmaceutical Research Vol 11 | Issue 2. P: 418:426.

    7. Amina M. Ibrahim and Sami M. Nasr (2019). Cytochrome oxidase subunit and internal transcribed spacer molecular revelation of infection with fasciola sp. in field- collected lymnaea natalensis snails. J. Egypt. Soc. Parasitol. (JESP), 49(2), 357 :364

    8. Shaaban R, El-Sayed WM, Samir S & El-Dabaa E (2019): Molecular and Biological Characterization of a Prepared Recombinant Human Interferon Alpha 2b Isoform. Applied Biochemistry and Biotechnology. 188 (1): 72-86

    9. Samar Ebrahim Abd El-Razek, Sabreen Mohamed El-Gamasy, Marwa Hassan, Mohamed S. Abdel-Aziz d, Sami Mohamed Nasr (2019). Transition metal complexes of a multidentate Schiff base ligand containing guanidine moiety: Synthesis, characterization, anti-cancer effect, and anti-microbial activity. Journal of Molecular Structure, Vol 1203, 127381.

2018

    1. Manal Diab, Mohamed Shemis, Doaa Gamal, Ahmed El-Shenawy, Maged El-Ghannam, Effat El-Sherbini, Mohamed Saber (2018). Helicobacter pylori Western cagA genotype in Egyptian patients with upper gastrointestinal disease. Egyptian Journal of Medical Human Genetics. 19, 297–300

    2. Manal Diab, Ahmed El-Shenawy, Mohamed Shemis, Maged El-Ghannam, Manal El Said, Moustafa Abdelnasser, Mahmoud Abdel-Hady, Effat El-Sherbini and Mohamed Saber (2018). Helicobacter pylori infection in egyptian patients with dyspepsia: diagnostic, demographic, endoscopic and clinical characteristics . Int. J. Adv. Res. 6(6), 226-234.

    3. Samah Mammdouh abdel-Hafiz, Hussam Mohamed Hamdy, Fatma EL-Zahraa Mohamed Khorshed, Tarek Salah El-Din Aboushousha, Gehan Safwat, Mohamed Ali Saber, Mohamed Ismail Seleem. (2018): Evaluation of Osteopontin as a Biomarker in Hepatocellular Carcinoma with Chronic HCV. Asian Pac J Cancer Prev, 19 (4), 1021-1027.

    4. Hala Farawela, Rabab Fouad, Nariman Zahran, Bothina Madkour, Zainab El-Saadany, Salaw Toimaa, Samah Mamdouh, Hisham El-Kiat. (2018): Fibronectin gene polymorphisms in HCV related type II mixed cryoglobulinemia: risk of development of B-cell lymphoma . Comparative Clinical Pathology . 27:1389–1395.

    5. Nasr SM, Ghareeb MA, Mohamed MA, Elwan NM, Abdel-Aziz AWA, Abdel-Aziz (2018) “MS. High-performance liquid chromatographyfingerprint analyses, In vitro cytotoxicity, antimicrobial and antioxidant activities of the extracts of two cestrum species growing in Egypt”. Phcog Res; 10:173-80.

    6.  Ghareeb M, Saad A, Ahmed W, Refahy L, Nasr S. (2018) “HPLCDAD- ESI-MS/MS characterization of bioactive secondary metabolites from Strelitzia nicolai leaf extracts and their antioxidant and anticancer activities In vitro“. Phcog. Res;10:368-78.

    7. Hala Badawi, Manal El Said, Safia Samir, Ahmed Ismail, Afkar Badawy, Ahmed Barakat. and Mohamed Saber (2018): Prevalence of Human Papillomavirus Genotypes among Egyptian Patients with Cancer Bladder. Journal of International Pharmaceutical Research. (45): 203-214.

    8. Hala Badaw, Mohammed Abass , Olfat Hamam , Manal Diab , Manal El Said , Ahmed Ismail, Gehan Mostafa , Safia Samir , Ehab El-Dabaa and Mohamed Saber (2018). Molecular and in-situ hybridiztion of human papiloma virus genotypes among Egyptian patients with bladder cancer. International journal of Pharmaceutical Research,10 (4): 402-411.

    9. Shaaban R, El-Sayed W M, & Samir S and El-Dabaa E (2018). Molecular and Biological Characterization of a Prepared Recombinant Human Interferon Alpha 2b Isoform. Applied Biochemistry and Biotechnology. https://link.springer.com/article/. DOI 10.1007/s12010-018-2908-y. ISSN 1559-0291.

2017

    1. Ahmed A. Hosni, A. Adel Abdel-Moneim , Eman S. Abdel-Reheim ,Samah M. Mohamed , Hamdi Helmy. (2017): Cinnamaldehyde potentially attenuates gestational hyperglycemia in rats through modulation of PPARɤ, proinflammatory cytokines and oxidative stress. Biomedicine & Pharmacotherapy, 88, 52-60.

    2. Tarek S. Aboushousha, Samah Mamdouh, Hussam Hamdy, Noha Helal, Fatma Khorshed, Gehan Safwat, Mohamed Seleem. (2017): Immunohistochemical and Biochemical Expressions Patterns of TTF-1, RAGE, GLUT-1 and SOX2 in HCV-Associated Hepatocellular Carcinoma. Asian Pac J Cancer Prev., 19(1), 219-227.

    3. Mohamed A Saber , Samah MM AbdelHafiz , Fatma E Khorshed , Tarek S Aboushousha , Hussam EM Hamdy , Mohamed I Seleem , Amira H Soliman (2017): Differential Expression of Glypican-3 and Insulin–Like Growth Factor-II mRNAs and Alpha-Fetoprotein and Ki-67 Markers in HCV Related Hepatocellular Carcinomas In Egyptian Patients. Asian Pacific Journal of Cancer Prevention. 18: 121-127.

    4. Samah Mamdouh, Fatma Khorshed, Tarek Aboushousha, Hussam Hamdy, Ayman Diab. Mohamed Seleem, Mohamed Saber. (2017): Evaluation of Mir-224, Mir-215 and Mir-143 as Serum Biomarkers of HCV Associated Hepatocellular Carcinoma. Asian Pac J Cancer Prev., 18(11), 3167-3171.

    5. Ahmed El-Shenawy, Manal Diab, Mohamed Shemis, Maged El-Ghannam, Dalia Salem, Moustafa Abdelnasser, Mohamed Shahin, Mahmoud Abdel-Hady, Effat El-Sherbini, Mohamed Saber (2017). Detection of Helicobacter pylori vacA, cagA and iceA1 virulence genes associated with gastric diseases in Egyptian patients. Egyptian Journal of Medical Human Genetics.

    6. Soliman R.H., Ismail O.A., Badr M.S., Nasr S.M. (2017) “Resveratrol ameliorates oxidative stress and organ dysfunction in Schistosoma mansoni infected mice” Experimental Parasitology 174, 52-58.

    7.  Huda R. M. Rashdan, Sami M. Nasr, Heba A. El-Refai, Mohamed S. Abdel-Aziz (2017) “A Novel Approach of Potent Antioxidant and Antimicrobial Agents Containing Coumarin Moiety Accompanied with cytotoxicity studies on the Newly Synthesized Derivatives” Journal of Applied Pharmaceutical Science. Vol. 7 (07), pp. 186-196.

    8. Mohammed Ali Saber, Safia Samir El-Naggar, Moatz Hassen Hassanein and Ahmed Barakat barakat. (2017): Role of MxA and Neutralizing antibodies in early response to Interferon treatment in Hepatitis C Egyptian Patients. European Journal of Biomedical and pharmaceutical Sciences. 4(2): 84-94.

    9. Ahmed El-Shenawy, Manal Diab, Mohamed Shemis, Maged El-Ghannam, Dalia Salem, Moustafa Abdelnasser, Mohamed Shahin, Mahmoud Abdel-Hady, Effat El-Sherbini and Mohamed Saber (2017). Detection of Helicobacter pylori vacA, cagA and iceA1 Virulence Genes Associated with Gastric Diseases in Egyptian Patients. Egyptian Journal of Medical Human Genetics. https://doi.org/10.1016/j.ejmhg.2017.04.003

    10. Mohamed Saber, Hend Okasha Ahmed, Magdy Mahmoud Mohamed and Mahmoud Hassan Romeih. (2017): Production of recombinant human interferon gamma by batch fermentation in E.coli system. Arab Journal of Biotechnology. Vol. 20: 11-19.

2016

    1. Ahmed Mostafa Aref , Mohamed Shamrouh Othman, Samah Mamdouh, Ehab Dabaa, Moataz Hassanein, Mohamed Ali Saber. (2016): New Biomarkers for Response to Treatment of HCV Infected Patients Based on IP-10 and IL 28B Polymorphism Analysis. American Journal of Biochemistry. doi:10.5923/j.ajb.20160605.02.

    2. Walaa Mosaad, Dalia Ibrahim Ramadan, Mohamed Abbas Shemis (2016). Prevalence of mutations within major hydrophilic region of hepatitis B virus and their correlation with genotypes among chronically infected patients in Egypt. Arab Journal of Gastroenterology 47 17(1).

    3. Eman El-Ahwany, Faten Nagy, Mona Zoheiry, Mohamed Shemis, Mona Nosseir, Hoda Abu Taleb, Maged El Ghannam, Rafaat Atta, Suher Zada (2016). Circulating miRNAs as Predictor Markers for Activation of Hepatic Stellate Cells and Progression of HCV-Induced Liver Fibrosis. Electronic Physician 8(1): 1804-1810.

    4. Eman El-Ahwany, Faten Nagy , Mona Zoheiry , Maged ELGhannam , Mohamed Shemis , Mohamed Aboul- Ezz , Suher Zada (2016). The Role of MicroRNAs in Response to Interferon Treatment in Chronic Hepatitis C patients. Electronic Physician, 8(2): 1994-2000.

    5. Manal Diab, Mohamed Shemis, Maged El-Ghannam , Doaa Gamal , Magda Azmy , Dalia Salem , Soheir Mansy and Mohamed Saber (2016). Helicobacter pylori vacA genotyping in relation to cagA status, ultra-structure of gastric mucosa and clinical outcomes in Egyptian patients. African Journal of Microbiology Research, 10 (14): 465-472.

    6. Omar A. Tahaa, Heba k. abdelhakim, Zainab Zakariaa, Mohamed Shemis and Alaa Barakat (2016). Hypolipidemic and Anti-fatty Liver Effects Exerted by Pomgeranate (Punica granatum L., Lythraceae) Peel Extract in HepG2 cell-line and high-fat diet induced mice. International Journal of Pharmacy and Pharmaceutical Sciences, (8) 6.

    7. Romeih M., Saber M., Mamdouh S., Khorshed F., Medhat A. (2016): Promising miRNA Biomarkers for Predicting the Response to the Interferon Combined therapy in HCV-4 Egyptian Patients. Arab J. Biotech., Vol.19,No. (2) July : 123-134.

    8. Saber Mohamed Ali, Mohamed Nadia Abdullah, Mohamed Eman Abd Al-Azeem and Okasha Hend Ahmed (2016). Isolation, molecular cloning and gene expression of human inerferon gamma (IFN-γ) gene in Escherichia coli. Journal of Pure and Applied Science. 54 (3): 1-7.

2015

    1. Samah Mamdouh, Ayman M. Metwally, Ahmed M. Aref, Hussein M. Khaled, Olfat Hammam, Mohamed A. Saber (2015): Bat-26 is Associated with Clinical Stage and Lymph Node Status in Schistosomiasis Associated Bladder Cancer. American Journal of Biochemistry. doi:10.5923/j.ajb.20150501.03.

    2. Samya El-shishtawy, Nevine Sherif, Emad Abdallh, Laila Kame, Mohamed A. Shemis, Abdel Aziz Ali Saleem, Haitham Abdalla, Hesham Gamal el Din (2015). Occult Hepatitis C Virus Infection in Hemodialysis Patients; Single Center Study. Electronic Physician, 7 (8): 1619-1625.

    3. Faten Z Mohamed, Mohamed A Shemis, Mona N Mohareb, Heba A Hosiny (2015). Studies on Nanoengineered Multilayer Capsules for Drug Delivery. Indian Journal of Novel Drug delivery, 7(2): 62-72.

    4. Saber M.A. , Moubasher M.H., Shaheen M.M., Mamdouh S. and Omar H. A. (2015): Human granulocyte colony stimulating factor (hG-CSF): cloning and expression in E. coli cells. Arab J. Biotech., Vol.18,No. (1) January : 45-54

    5. Sami M. Nasr, Mohamed K. Ibrahim, Hanaa A. Hammad, Hanan A. Omar, J.H. David Wu, Ryan Severins, Mohamed A. Saber (2015) “ Cloning and expression of Human Growth Hormone in E. coli System. Arab Journal of Biotechnology Vol. 18(1) January, P: 33-44.

2014

    1. Mona N. Moharib, Olfat A. Hammam, Fatma H. Salman, Mohammed M. El-naggar and Soad A. Sherif (2014): Transplantation of modified hepatocyte reduces hepatotoxicity induced by carbon tetrachloride. Life Science Journal. 11(8): 641-652.

    2. SaraMohamed Massoud, Mohamed Abbas Shemis, Ehab Aly Drees, Mamdoh Ahmed Taha, Hanan Abdelmoniem Omar, Mohamed Ali Saber (2014). Predicative Factors For Pegylated Interferon-Α Plus Ribavirin Combination Therapy In Egyptian Patients With Chronic Hepatitis C infected With Genotype 4. The Egyptian Journal of Biochemistry & Molecular Biology, 32 (N.2) 132-153.

    3. Soad A. Sherif , Mona N. Moharib, Naglaa M. El-lakkany, Olfat A. Hammam ,Fatma H. Salman and Mohammed M. El-naggar (2014): the use of microencapsulated hepatocytes transplantation reduces mortality and liver alteration in schistosoma mansoni infected hamsters. Journal of the Egyptian society of parasitiology. 44(1): 229-242.

2013

    1. SaberM, Diab T, Hammam O, Karim A, Medhat A, Khela M and El-Dabaa E (2013). Protective and Anti-Pathology Effects of Sm Fructose-1,6- Bisphosphate aldolase-based DNA vaccine against Schistosoma mansoni by changing route of injection. Korean J Parasitol., 51: 155-163.

    2. El Ray A, Asselah T, Moucari R, El Ghannam M, Taha AA, Saber MA, Akl M, Atta R, Shemis M, Radwan AS, Ghali A, Paradis V, Marcellin P. (2013). Insulin resistance: a major factor associated with significant liver fibrosis in Egyptian patients with genotype 4 chronic hepatitis C. Eur J Gastroenterol Hepatol., 25:421-7.

    3. Fam N, Gamal D, El Said M, Aboul-Fadl L, El Dabaa E, El Attar S, Sorur A, Fouad S and Klena J (2013). Detection of plasmid-mediated AmpC beta-lactamases in clinically significant bacterial isolates in a research institute hospital in Egypt. Life Science Journal, 10: 2294 – 2304.

    4. Fam N, Gamal D, El Said M, El Defrawy I, El Dabaa E, El Attar S, Sorur A, Ahmed S and Klena J (2013). Prevalence of plasmid-mediated ampC genes in clinical isolates of Enterobacteriaceae from Cairo, Egypt. British Microbiology Research Journal, 3: 525-537.

    5. Saad El Dine S, El Said M, El-Dabaa E, Abdel Raoof E, Raafat M, Nabil M, Hassanein M, Saber M, Omer M (2013). Occult Hepatitis B Virus Infection in Haemodialysis Egyptian Patients with Chronic Hepatitis C. Life Science Journal, 10: 237-245.

    6. Diab M, Fam N, El-Said M, El-Dabaa E, El- Defrawy I and Saber M (2013). Occurrence of VIM-2 Metallo-ß- Lactamases in imipenem resistant and susceptible Pseudomonas aeruginosa clinical isolates from Egypt. African Journal of Microbiology Research, 7: 4465-4472.

    7. Ahmed El Ray, Tarik Asselah, Rami Moucari, Maged El Ghannam, Alaa A. Taha, Mohamed A. Saber, Maha Akl, Raafat Atta, Mohamed Shemis, Azza S. Radwan, Ayman Ghali, Valerie Paradis and Patrick Marcellin (2013). Insulin Resistance: A Major Factor Associated With Significant Liver Fibrosis in Egyptian Patients with Genotype 4 Chronic Hepatitis C. European Journals of Gastroenterology & Hepatology 25 (4): 421-427.

    8. Saber M A, Omar H A, Shemis MA, El-Dabaa E, Hassan HM, Moharib MN, Abou-Shousha TS and Romeih MH (2013). Evaluation of the immune response induced by DNA vaccine cocktail expressing Schistosoma mansoni genes. Arab Journal of Biotechnology, 15: 157-172.

2012

    1. Aitta AA, El Said M, El Dabaa E, El Salam MA and Hesham MM (2012). Detection of Plasmid-Mediated 16S rRNA Methylase Conferring High-Level Resistance to Aminoglycosides in Gram negative bacilli from Egypt. Life Science Journal. 9 (4 ): 2472-2479.

    2. Khattab RA, Rasmy SA, Ragab YM, Said DG, Abdelfatah MM, Shemis MA, Ezzat DM and Abdel Rahman FS (2012). Evaluation of Polymerase Chain Reaction and Culture for the Diagnosis of Corneal Ulcer. Life Science Journal, 9:746-755.

    3. Shemis MA, El-Abd DM, Ramadan DI, Mohamed, El-Sayed I, Guirgis BS, Saber MA, Azzazy HM. (2012). Evaluation of Multiplex Nested Polymerase Chain Reaction for Routine Hepatitis C Virus Genotyping in Egyptian Patients. Hepat Mon., 12:265-70.

    4. Rania A. Khattab, Salwa A. Rasmy, Yasser M. Ragab, Dalia G. Said, Maha M. Abdelfatah, Mohamed A. Shemis, Dalia M. Ezzat and Fatma Elzahraa S. Abdel Rahman (2012). Evaluation of Polymerase Chain Reaction and Culture for the Diagnosis of Corneal Ulcer. Life Science Journal 9(3)746-755.

    5. Evaluation of the immune response induced by DNA vaccine cocktail expressing Schistosoma mansoni genes (2012). Saber M. A., OmarH. A., ShemisM. A., El-Dabaa E., HassanH. M., MoharibM. N., Abou-Shousha T.S. and RomeihM. H.ArabJ.Biotech.Vol.15, No. (2):157-172.

2011

    1. Xie Q, Wondergem R, Shen Y, Cavey G, Ke J, Thompson R, Bradley R, Daughtery-Holtrop J, Xu Y, Chen E, Omar H, Rosen N, Wenkert D, Xu HE, Vande Woude GF (2011). Benzoquinone ansamycin 17AAG binds to mitochondrial voltage-dependent anion channel and inhibits cell invasion. Proc Natl Acad Sci U S A. 108:4105-10.

    2. Mostafa MS, El-Seidi EA , Kassem A, Shemis M, Saber M, Michael MN. (2011). Detection of ascitic fluid infections in patients with liver cirrhosis and ascites. Arab Journal of Gastroenterology, 12:20–24.

    3. Almahdy O, EL-Fakharany EM, EL-Dabaa E, Tzi Bun Ng , Redwan EM (2011). Examination of the Activity of Camel Milk Casein against Hepatitis C Virus (Genotype-4a) and Its Apoptotic Potential in Hepatoma and HeLa Cell Lines. Hepat Mon, 11:724-730.

    4. Saber M, Essmat G, Shaaban R, Khalifa MH and El-Dabaa E (2011). Incidence of anti- Interferon-α2 antibodies in Egyptian HCV infected patients treated with peg-IFN-α2 and Influence on Response to treatment. New Egyptian Journal of Medicine, 44: 30-38.

    5. Saber M.A, Abdallah SO, Mabrouk ME, El Sehrawy FE , Shaheen M and Romeih MH (2011). Identification of genotypes and subtypes of Hepatitis C Virus in the Blood of Egyptian infected patients. Arab J Biotech, 14:1-12

2010

    1. Badawi H, Ahmed H, Aboul Fadl L, Helmi A, Fam N, Diab M, Ismail A, Badawi A, Saber M (2010). Herpes simplex virus type-2 in Egyptian patients with bladder cancer or cystitis. Acta Pathologica, Microbiologica, Et Immunologica Scandinavica [APMIS],118: 37-44.

    2. Elattar G, Saleh Z, EL-Shebini S, Farrag A, Zoheiry M, Hassanein A, EL-Ghannam M, Shendy S, EL-Dabaa E, Zahran N (2010). The use of whey protein concentrate in management of chronic hepatitis C virus – a pilot study. Arch Med Sci., 6 : 748- 755.

    3. Saber M., Hammad H., El-sherif S., Shabaan R., Nassar M, Nasr S., Okasha H., El-Dabaa E. (2010): “Lab scale production of recombinant pharmaceutical proteins established skills for R&D and innovative research” 3rd Stakeholders Meeting of the African Network for drugs and Diagnostics Innovation 11-13 October 2010 United Nations Office in Nairobi UNON, Kenya.p:119

    4. Ghreeb A., Saber M. A., Nasr S.,”Cloning of Human growth hormone HGH in TA cloning vector (2010): 5th Scientific Environmental Conference Faculty of Science, Zagazig University 19 may. P: 49:58

    5. Herpes simplex virus type-2 in Egyptian patients with bladder cancer or cystitis (2010). Badawi H, Ahmed H, Aboul FadlL, Helmi A, Fam N, Diab M, Ismail A, Badawi A, Saber M. APMIS. 118(1):37-44.