Immunology Department

   In the near future, plans for the Department include:

• Immunodiagnosis of endemic diseases and their consecutive complications by the development of immunodiagnostic kits with high quality. We produce our own monoclonal antibodies against various endemic infections, and employ them in different diagnostic techniques such as sandwich ELISA, nanoparticle-modified sandwich ELISA, and gold nanoparticle immunostrip assay.
• Tissue engineering and stem cell research.
• Production of cell lines and their implementation in assessment of cytotoxicity of different anticancer compounds.
• Investment of molecular biology techniques in the genetic and epigenetic fields for diagnosis, monitoring and therapeutic approaches.
• Immunochemistry and its central role in characterization and purification of our diagnostic reagents (monoclonal and polyclonal antibodies).
• Utilization of nanotechnology in diagnosis and therapy.
• Establishment of a Biobank for samples of Egyptian patients having liver diseases.
• Bioinformatics: A field that develops methods and software tools and combines Biology, Computer Science, Information Engineering, Mathematics, and Statistics to analyze and interpret the biological data.
• Capacity building by offering training courses for Egyptian and African researchers interested in our developed techniques: MAb production, Stem cell culture, and all forementioned techniques.

The mission of the Immunology Department is the development of a research environment in which a broad range of interdisciplinary work is carried out to cope with the new era of the Immunology, stem cell biology, Bioinformatics, and Biobanking.

In particular, the Department targets the diagnosis and management of endemic, liver, Gastrointestinal, urological, and pandemic diseases.

Associate Prof.  Marwa Hassan Mohammed
[email protected]

Former Heads 

Emeritus Prof. Zeinab Ahmed Shaker

Emeritus Prof. Hanaa Ismail Hassanein

Late Prof. Azza El Bassiouny

Emeritus Prof. Zeinab Abou Bakr Demerdash

Emeritus Prof.  Kesmat Mohamed Maher

Emeritus Prof. Faten Salah El-Din Mahmoud

Emeritus Prof. Faten Moustafa Nagy

Prof. Eman Gamal El-Din El-Ahwany

Staff Members 

Emeritus Prof. Moustafa Abdel Haleem Kadah

Emeritus Prof. Zeinab Ahmed Shaker

Emeritus Prof. Hanaa Ismail Hassanein

Late Prof. Azza El Bassiouny

Emeritus Prof. Hanan Mohamed Gamal El-Din El-Baz

Emeritus Prof. Zeinab Abou Bakr Demerdash

Emeritus Prof.  Mohamed Youssef Mohamed El-Mohandes

Emeritus Prof.  Manal Mohamed Kamel

Emeritus Prof.  Kesmat Mohamed Maher

Emeritus Prof.  Salwa Hassan Mohamed

Emeritus Prof. Faten Salah El-Din Mahmoud

Emeritus Prof.  Mona Mohamed Kamel Zoheiry

Emeritus Prof.  Wafaa Abd El Fattah Mansour

Prof. Eman Gamal El-Din El-Ahwany

Emeritus Prof. Faten Moustafa Nagy

Associate Prof.  Marwa Hassan Mohammed

Associate Prof.  Shimaa Attiah Hassan

Researcher Sarah Maher

Researcher  Mohamad Hussien Elzallat

Associate Researcher Engy Mohsen Abdel-Moniem

Associate Researcher Dina Samir Abdel-Hady

Associate Researcher Noha Mohamed Abd El Rahman

Associate Researcher  Sherihan Mohammed Al Motawakel

Late Resident Shimaa Abd El Sattar Abbas

Resident Hadeel Khaled Mohammed

Resident Rawan Mohsen Mousa

Resident Arwa Mansy

Resident Lamia Madkour

Resident Amany Rezk

Resident Mohamed Hatem

Resident Afnan Mohamed Sarhan

The strategy of  Immunology Department constitutes an integrated part of the general strategy of TBRI, targeting immunodiagnosis of endemic diseases (viral and parasitic), assessment of immune response to endemic infections and their associated morbidity changes, and tackling immunological approaches for management of endemic diseases and their hepatic and urinary complications with special stress on the following targets:

Immunodiagnosis of endemic diseases and their consecutive complications:

1. Immunodiagnosis of parasitic infections:

• The detection of specific circulating anti- parasitic IgG4 improved sensitivity and specificity of immunodiagnosis of schistosomiasis, fascioliasis¹ and filariasis, and can be used as screening test in endemic areas.
• The use of affinity purified fractions of parasitic antigens e.g. a Con-A purified hydatid glycoprotein fraction, and affinity purified Fasciolaworm antigen fraction, improved immunodiagnosis of human hydatidosis and fascioliasis.
• Production of MAbs against parasitic Antigens:

–  S. mansoni soluble egg Ag

–  S. haematobium soluble egg Ag

–  S. mansoni worm tegument Ag

–  F. gigantica crude worm Ag

–  F. gigantica ex/sec Ag

–  W. bancrofti worm extract

• Establishment of antigen detection immunoassays (sandwich ELISA, dot ELISA and latex agglutination test) using our MAbs as diagnostic probes for circulating parasitic antigens in patients’ sera and/ or excreta (urine or stool). 
• Development of antigen detection kit for diagnosis of active schistosomiasis and monitoring of cure on pre-commercial level. 

1. Immunodiagnosis of hepatitis viruses.
2. Tissue typing:

The feasibility of using the following immunomolecular markers and biomarkers as predictive or prognostic markers for HCC:

1. Evaluation of the efficacy of medicinal herbs or drugs on malignant cell lines.

Predication of malignant transformation:

1. Assessment of Genetic, epigenetic, and protein markers for early diagnosis and prognosis of patients having liver, GIT, and urological malignancies.
2. Genotyping of patients to predict those who might develop health disorders.

Immunotherapy:

1. Establishment of tissue engineering unit for stem cell culture and   engraftment into animal models and patients as a new line for management of liver cell failure.
2. Myoblast cell culture as a preliminary step for their engraftment in bladder cervix sphincter as a replacement therapy for urine incontinence.
3. Establishment of a cord blood stem cell bank as a source of allogenic stem cells for treatment of complications of endemic diseases.
4. Establishment of a defined protocol for in vitro trans-differentiation of cord blood (CB) unrestricted somatic stem cells (USSCs) into hepatocyte-like cells.
5. Induction of hepatic regeneration in an experimental model using the hepatocyte–like cells differentiated from in vitro cultured CB USSCs.
6. Establishment of the use of CB-USSCs as a future candidate for cellular therapy of hepatic failure on experimental level. 
7. Gene therapy.

• Establishment of a biobank for samples of Egyptian patients having liver diseases.
• Vaccination

1)  Immunodiagnostics laboratory 

• Diagnosis of Parasitic Infection (ELISA):
• Detection of circulating anti-schistosome antibodies.
• Detection of circulating schistosome antigen.
• Detection of circulating anti-Fasciola hepaticaantibodies.
• Detection of circulating anti-E. granulosusantibodies.
• Detection of Autoantibodies: 
• Anti-nuclear antibodies.
• Autimmune Abs in autoimmune liver diseases.
• Detection of Hepatitis Markers.
• Detection of HB surface antigen.
• Detection of circulating anti-HCV antibodies.
• Detection of HIV Abs.
• Enzyme-linked immune-electrophoresis transfers blot (EITB).
• SDS-polyacrylamide gel electrophoresis (SDS- PAGE).
• Immunodiffusion.
• Immunofluorescence.
• Immunohistochemistry and immunolocalization.

2)  Tissue engineering units:

• Isolation of different types of stem cells (MSCs & USSCs) from bone marrow and cord blood. 
• Stem cells characterization by flowcytometry, and gene expression analysis by real-time PCR.
• Induction of differentiation of stem cells into osteogenic, chondrogenic and adipogenic lineages.
• Experimental treatment of hepatic fibrosis (induced by CCl4 and Schistosoma infection) by stem cells. 

3)  Monoclonal antibody production unit:

• Tissue culture techniques:
• Basics of tissue culture.
• Monoclonal antibody production by hybridoma technology against different parasitic antigens (Schistosoma mansoni, Schistosoma haematobium, Fasciola, Filaria, and Hydatid). 
• Characterization of monoclonal antibody:
•  Isotype characterization.
•   SDS and Immunoblotting.
•   Polyclonal antibody production in rats.
•  Mononuclear cell separation. 

Purification:

•    Ammonium sulfate.
•   Boric acid.
•   Conjugation of MAb by HRP.
•  Development of immunodiagnostic kits (ELISA) for endemic diseases using our own monoclonal antibodies. 

4)  Molecular Biology unit:

• Basics of molecular biology.
•   RNA, DNA and microRNA extraction.
• DNA hypo/ hyper-methylation.
•    Gene expression analysis by real-time PCR.

•  Detection of epigenetics markers using real-time PCR.

5)  Experimental animal unit:

Establishment of HCC murine model.

Laboratory Structure and Facilities

The Immunology Department consists of a Central Immunology Research Laboratory and a “Tissue Culture Unit”.

The Central Immunology Research Laboratory consists of the following units:

–  Immunodiagnostics laboratory (ELISA, immunofluorescent technique, agglutination tests, etc.).

–  Immunochemistry laboratory (electrophoresis, immunoelectrophoresis, SDS-PAGE, immunoblotting, Isoelectric focusing, PCR, affinity and ion-exchange chromatography, etc.).

–   The Tissue Culture Unit includes:

        A preparatory laboratory.

        Tissue engineering units.

        GMP unit for stem cells.

        Monoclonal production unit.

–         Molecular unit includes:

• High speed cooling centrifuge.
• Thermal cycler.
• Real-time PCR.

List of the most important equipment:

–      Laminar flow cabinets.

–      CO2 incubators.

–      Liquid nitrogen containers and controlled rate freezing apparatus.

–      Microscopes (inverted and fluorescent).

–      DNA and protein electrophoresis and electroelution systems.

–      Transblot cell. 

–      PCR apparatus.

–      UV trans-illuminator and UV camera.

–      ELISA reader.

–      Autoclave, ovens, incubators, shaking incubators and water baths.

–      Vertical and horizontal deep freezers (-20 degrees Celsius and -40 degrees Celsius).

–      Centrifuges (cooling and high speed centrifuge).

–      Bi-distillator.

–      Digital balances.

–      Electric tissue homogenizer, sonicator and lyophilizer.

–      Two computers are available for research work at the Immunology Department.

Fields of training:

–  Monoclonal antibody production.

–  Molecular immunology.

– Tissue culture.

– Stem cells.

– Epigenetic field.

-Serodiagnostic techniques (ELISA, Immunoblotting).

The Immunology Department at Theodor Bilharz Research Institute is considered as one of the Centers of Excellency for the production of monoclonal antibodies and their application in immunoassays for early diagnosis of active parasitic infection and monitoring of cure.

The research team at the Immunology Department, TBRI, is highly qualified and has vast experience and skills in the field of immunodiagnosis, characterization and purification of proteins, cell cloning, production, characterization and applications of monoclonal antibodies in immunoassays. Several international projects with relevant technologies have been carried out at the Immunology Department during the last few years.

Scope of Services 

• Diagnosis of Parasitic Infection (ELISA)
• Detection of circulating anti-schistosome antibodies.
• Detection of circulating schistosome antigen.
• Detection of circulating anti-Fasciola gigantica
• Detection of circulating anti- granulosusantibodies.

Detection of Autoantibodies

• Anti-nuclear antibodies. (ELISA).
• Anti-mitochondrial antibodies.
• Liver/kidney microsomal antibodies.
• LC-1 (cytosolic liver antigen type 1) antibodies.
• Anti-Soluble Liver Antigen/Liver-Pancreas (SLA/LP) Antibodies

Detection of Hepatitis Markers (ELISA)

• Detection of HB surface antigen.
• Detection of circulating anti-HBc antibodies (IgM & IgG).
• Detection of HBe antigen.
• Detection of circulating anti-HBe antibodies. 
• Detection of anti-HBs antibodies.
• Detection of circulating anti-HCV antibodies.

The Project of the Immunology Staff Members in the Last Ten Years is the Followings Projects:

1. Nationally and internationally funded projects:

• Novel point of care test for rapid diagnosis of covid 19 using recombinant nanobodies against SARS-CoV-2 Spike1 (S1) protein PI: Prof. Manal Kamel (2021-2022), funded by STDF.
• Development of MAb labeled nanoparticles-based immunostrip assay for early detection of bladder cancer biomarkers in Egyptian patients. PI: Prof. Manal Kamel (2020-2022), funded by Academy of Scientific Research and Technology, Science and Technology Center (STC), National Strategy for Genetic Engineering and Biotechnology (Phase iii: Applications & Products). 
• TBRI Biobank for Liver Diseases: Research Translation and Precision Medicine in Hepatology. PI: Mona Zoheiry (2020-2023), funded by STDF, STDF Grants for Accredited Centers of Scientific Excellence (STDF-ACSE).
• Epigenetic regulation of circulating cancer stem cell in HCV-induced hepatocellular carcinoma. PI: Mona Zoheiry (2020-2022), funded by STDF, Basic and Applied Research Grant (BARG), Call 6.
• Polymer Nanoparticle Mediated Delivery of MicroRNA: A Novel Potential Therapeutic Tool for Hepatocellular Carcinoma in an Experimental Model. PI: Prof. Eman El-Ahwany (2019-2021), funded by Science Technology Development Fund (STDF), Basic and Applied Research Grant (BARG), Call 4.
• Long Non-Coding RNA: An Innovative Therapeutic Tool for Hepatocellular Carcinoma in vitro and in vivo. PI: Prof. Eman El-Ahwany (2019-2021), funded by STDF, Innovative Grant, Call 6.
• Development of easy to use and affordable biomarkers as diagnostics for types II and III diseases. PI: Prof. Dr. Hanan El-Baz (2016 – 2019), funded by World Health Organization (WHO).
• Project Based Training and Fellowship on Development, Production and Access to Diagnostics. PI: Prof. Dr. Hanan El-Baz  (2015 – 2019), funded by African Network for Drug & Diagnostic Innovation (ANDI).
• The differentiation potential of human cord blood- derived mesenchymal stem cells into functional hepatocyte-like cells on nanofibrous scaffold. ID: 12 Biotechnology, PI: Prof. Zeinab Demerdash, (2014-2018), funded by TBRI.
• The Generation of Cord Blood-Derived Unrestricted Somatic Stem Cells and Their Differentiation into Hepatocyte-like Cells Pave the Way for Liver Regeneration.  PI: Prof. Zeinab Demerdash, (2010-2015), funded by STDF (Target call, HCV Call).

1. TBRI funded projects:

• Circulating cancer stem cells markers: Diagnostic tools in chronic/cirrhotic viral hepatitis patients. ID: 107 Diagnosis, PI: Dr. Marwa Hassan (2018-2020).
• Potential effect of bone marrow-derived stem cells for treatment of experimentally induced ulcerative colitis in animal model. ID: 125 Therapy, PI: Dr. Shimaa Attia (2018-2020).
• Potential effect of bone marrow-derived stem cells for treatment of experimentally induced ulcerative colitis in animal model. ID: 125 Therapy, PI: Dr. Shimaa Attia (2018-2020).
• Circulating MicroRNAs as Diagnostic and Prognostic Tools in HCV-induced Liver Fibrosis. PI: Prof. Mona Zoheiry (2017-2019).
• Expansion of anti-Schistosomamansoni secretory hybridomas in chemically defined serum free culture as a reliable source for monoclonal antibodies. ID: 27 Biotechnology, PI: Prof. Kesmat Maher (2018-2020.
• Effect of repeated passaging and cell density on proliferation and differentiation potential of cord blood Mesenchymal stem cells.  ID: 11 Biotechnology, PI: Prof. Kesmat Maher, (2014- 2018).
• The differentiation potential of human cord blood- derived mesenchymal stem cells into functional hepatocyte-like cells on nanofibrous scaffold. ID: 12 Biotechnology, PI: Prof. Zeinab Demerdash, (2014-2018).
• Transplantation of Hepatogenic Differentiated Mesenchymal Stem   Cells versus Adult Hepatocytes in Experimental Chronic Liver Insult Model: A Comparative Study. ID: 13 Biotechnology, PI: Prof. Hanan El-Baz, (2013-2018).
• Prediction of miRNA Target Genes Involved in Liver Cancer Pathways and its Validation. ID: 14 Biotechnology, PI: Prof. Faten Nagy (2014-2019),.
• Mesenchymal stem cells with or without targeted gold coated magnetic nanoparticles as a novel therapeutic tool in hepatocellular carcinoma murine model: A pilot study. ID: 19 Biotechnology, PI: Prof. Wafaa A. Mansour (2014-2019).
• Novel Diagnostic and Therapeutic Tools in Murine Model of Hepatocellular Carcinoma: A Pilot Study. ID: 8 Biotechnology, PI: Prof. Eman El-Ahwany (2012-2017).
• Anticancer Potential of Some Medicinal Herbs on both Hepatocellular Carcinoma and Squamous Cell lines. ID: 98 Therapy, PI: Prof.  Hanaa Hassanein, (2012-2015).
• Circulating markers related to influx of the liver with extrahepaticmyo-fibroblast progenitor cells as predictors of HCV-induced hepatic fibrosis and carcinogenesis. ID: 86 Diagnosis, PI: Prof. Mona Zoheiry (2011-2017).
• Hepatogenic Differentiation Potentials of Human Mesenchymal   Stem Cells: An in-vitro Study. ID: 5 Biotechnology, PI: Prof. Hanan El-Baz (2010-2018).
• Processing and Cryopreservation of Cord Blood: A preliminary Step Towards the Establishment of TBRI National Stem Cell Bank. ID: 3 Biotechnology, PI: Prof. Zeinab Demerdash (2009-2014).
• Autologus Hematopoietic Stem Cells in The Treatment of End-Stage Liver Disease: A Pilot Clinical Trial conducted in TBRI. ID: 2 Biotechnology, PI: Prof. Hanan El-Baz (2009-2015).
• Culture of hybridoma cell lines in serum- free media using spinner flasks: A step forward for large scale production of monoclonal antibodies of high quality. ID: 4 Biotechnology, PI:  Prof. Faten Salah, (2009-2012).
• Processing and Cryopreservation of Cord Blood: A preliminary Step Towards the Establishment of TBRI National Stem Cell Bank. ID: 3 Biotechnology, PI: Prof. Zeinab Demerdash (2009-2014).
• Pilot Study of Anticancer Potential of Some Herbal Medicine. ID: 89 Therapy, PI: Prof. Hanaa Hassanein (2008-2010).

Cooperative Projects

• Prognostic value of immune biomarkers (PD-1/PD-L1 and CTLA4) in patients with bladder carcinoma with/without Schistosomiasis. Project NO: 118 Diagnosis, Participant: Dr. Marwa Hassan (2020 till now), funded by TBRI.
• A novel nanotechnology -based approach for the rapid diagnosis of infectious diseases using nano-chip. PI: Prof. Dr. Ibrahim Rabie (Parasitology Department), Participant: Dr. Shimaa Attia (2020-2022), funded by STDF, Innovative Grant, Call 6.
• The antistress ability of medicinal plants on cultured stem cells. PI: Asmaa Salah (Medicinal Chemistry Department), Dr. Shimaa Attia (2020-2022), funded by TBRI.
• Water borne diseases risk associated with human activities in River Nile in the area of Greater Cairo with special emphasis on Scistosomiasis, Escherichia coli, Cryptosporidium and Giardia. ID: 104 Control, PI: Prof. Hanaa EL-Khiat (Environmental Research Department), Participant: Dr. Shimaa Attia (2018-2020), funded by TBRI.
• Preliminary study of the prevalence of NAFLD/NASH in Egyptian patients with T2DM. Project NO: 123 Therapy, Participant: Prof. Dr. Mona Zoheiry, Prof. Dr.  Eman El-Ahwany, Dr. Marwa Hassan (2018 till now), funded by TBRI..
• Assessment of potential synergistic or antagonistic toxicity mechanisms during co–exposition of in vitro models towards cerium dioxide nanoparticles and environmental chemicals/ pharmaceuticals. PI: Prof. Mohamed Shemis (TBRI), Participants: Prof. Eman El-Ahwany and Dr. Marwa Hassan (2018-2020), funded by the German Egyptian Research Fund (GERF).
• DNA Methylation and microRNA Crosstalk as epigenetic Prognostic Biomarker for HCV Induced Hepatocellular Carcinoma. ID: 24 Biotechnology, PI: Prof. Faiza El-Essawy, (Hematology Department), Participant: Prof: Mona Zoheiry (2017-2019), funded by TBRI.
• Long non-coding RNA as a novel non-invasive diagnostic tool for HCV-induced hepatocellular carcinoma. ID: 23 Biotechnology, PI: Prof. Faiza El-Essawy (Hematology Department), Participant: Prof. Eman El-Ahwany (2017-2019), funded by TBRI.
• SAT-2, SAT-3, ALU and LINE1 hypo methylation as an early diagnostic and prognostic marker for HCV related hepatocellular carcinoma. ID: 100 Diagnosis, PI: Prof. Moataz Siam (Gastroenterology Department), Participants: Prof. Eman El-Ahwany & Dr. Marwa Hassan (2015-2017) funded by TBRI.
• Unrecognized chronic liver diseases (CLD): Impact on diagnosis, treatment and disease progression of CLD in adult Egyptian patients. Project NO: 92 Diagnosis, Participants: Prof. Eman El-Ahwany & Dr. Marwa Hassan (2014-2020), funded by TBRI.
• MiRNAs as a novel therapeutic tool in HCV-induced liver fibrosis. PI: Prof. Suher Zada (AUC), CO-PI: Prof. Eman El-Ahwany (2011-2012), funded by the American University in Cairo (AUC).
• Theodor Bilharz into European research Area” THEBERA” project. PI: Sanaa Botros, Participant: Prof. Hanan El-Baz (2010-2015), funded by European Union (EU), as an ERA-WIDE FP7.
• Nanoparticles as a Delivery System for Targeting Schistosmiasis and Fascioliasis: A pilot study.  PI: Prof. Suher Zada (AUC) & Prof: Ibrahim Rabie (TBRI), CO-PI: Prof. Eman El-Ahwany. Funded by the Science Technology Research Centre (STRC), the American University in Cairo and TBRI, (2010-2011).
• A therapeutic trial for HCV infection using small interfering RNA (siRNA) targeting the viral genome.  PI: Prof. Mahmoud El Hefnawi (National research Centre), Participant: Prof. Eman El-Ahwany (2010-2014), funded by Technology Development Fund (STDF), Target Call HCV. 

Publications in the Last Ten Years

Mohamed Nasr, Simona Cavalu, Sameh Saber, Mahmoud E Youssef, Amir Mohamed Abdelhamid, Heba I Elagamy, Islam Kamal, Ahmed Gaafar Ahmed Gaafar, Eman El-Ahwany, Noha A Amin, Samuel Girgis, Rawan El-Sandarosy, Fatma Mahmoud, Hadeer Rizk, Merna Mansour, Amal Hasaballah, Amira Abd El-Rafi, Reem Abd El-Azez, Mahy Essam, Dina Mohamed, Nada Essam, Osama A Mohammed  (2022). Canagliflozin-loaded chitosan-hyaluronic acid microspheres modulate AMPK/NF-κB/NLRP3 axis: A new paradigm in the rectal therapy of ulcerative colitis. Biomedicine & Pharmacotherapy, 153: 113409.

Ahmed A Shaaban, Amir Mohamed Abdelhamid, Mohamed E Shaker, Simona Cavalu, Adrian Marius Maghiar, Abdulrahman A Alsayegh, Ahmad O Babalghith, Eman El-Ahwany, Noha A Amin, Osama A Mohammed, Hanan Eissa, Ahmed Gaafar Ahmed Gaafar, Gaber El-Saber Batiha, Sameh Saber (2022). Combining the HSP90 inhibitor TAS-116 with metformin effectively degrades the NLRP3 and attenuates inflammasome activation in rats: A new management paradigm for ulcerative colitis. Biomedicine & Pharmacotherapy, 153: 113247.

AM Abdelhamid, ME Youssef, S Cavalu, G Mostafa-Hedeab, A Youssef, ST Elazab, S Ibrahim, S Allam, RM Elgharabawy, E El-Ahwany, NA Amin, A Shata, OA Mohammed, MS Ibrahim Abdeldaiem, A Alhowail, G El-Saber Batiha, EA El-Mahmoudy, M Attia, A Allam, MY Zaater, MM Osman, M Nader, A Taha, NA Makarem, S Saber (2022). Carbocisteine as a Modulator of Nrf2/HO-1 and NFκB Interplay in Rats: New Inspiration for the Revival of an Old Drug for Treating Ulcerative Colitis. Frontiers in Pharmacology, 13: 887723. 

Sameh Saber, Mohamed Nasr, Mohamed MY Kaddah, Gomaa Mostafa-Hedeab, Simona Cavalu, Ahmed AE Mourad, Ahmed Gaafar Ahmed Gaafar, Sameh S Zaghlool, Safaa Saleh, Mohamed M Hafez, Samuel Girgis, Rehab Mohamed Elgharabawy, Karim Nader, Mansour Alsharidah, Gaber El-Saber Batiha, Eman El-Ahwany, Noha A Amin, Heba I Elagamy, Ahmed Shata, Reem Nader, Ahmed E Khodir (2022). Nifuroxazide-loaded cubosomes exhibit an advancement in pulmonary delivery and attenuate bleomycin-induced lung fibrosis by regulating the STAT3 and NF-κB signaling: A new challenge for unmet therapeutic needs. Biomedicine & Pharmacotherapy, 148: 112731.

Mona H Zohny, Simona Cavalu, Mahmoud E Youssef, Mohamed MY Kaddah, Ahmed AE Mourad, Ahmed Gaafar Ahmed Gaafar, Eman El-Ahwany, Noha A Amin, Heba M Arakeep, Ahmed Shata, Safaa Saleh, Mohamed M Hafez, Sara T Elazab, Rasha Abdelhady, Rehab Mohamed El Shahat, Galal Yahya, Sameh Saber (2022). Coomassie brilliant blue G-250 dye attenuates bleomycin-induced lung fibrosis by regulating the NF-κB and NLRP3 crosstalk: A novel approach for filling an unmet medical need. Biomedicine & Pharmacotherapy, 148: 112723.

Eslam E Abd El-Fattah, Sameh Saber, Ahmed AE Mourad, Eman El-Ahwany, Noha A Amin, Simona Cavalu, Galal Yahya, Ahmed S Saad, Mansour Alsharidah, Ahmed Shata, Haidy M Sami, Mohamed MY Kaddah, Amal MH Ghanim (2022). The dynamic interplay between AMPK/NFκB signaling and NLRP3 is a new therapeutic target in inflammation: Emerging role of dapagliflozin in overcoming lipopolysaccharide-mediated lung injury. Biomedicine & Pharmacotherapy, 147: 112628.

EE Abd El-Fattah, S Saber, ME Youssef, H Eissa, E El-Ahwany, NA Amin, M Alqarni, GES Batiha, AJ Obaidullah, MMY Kaddah, AG Ahmed Gaafar, AAE Mourad, G Mostafa-Hedeab, AM Abdelhamid (2022). AKT-AMPKα-mTOR-dependent HIF-1α Activation is a New Therapeutic Target for Cancer Treatment: A Novel Approach to Repositioning the Antidiabetic Drug Sitagliptin for the Management of Hepatocellular Carcinoma. Frontiers in Pharmacology, 12: 720173.

Amir Mohamed Abdelhamid, Sameh Saber, Mahmoud E Youssef, Ahmed Gaafar Ahmed Gaafar, Hanan Eissa, Marwa A Abd-Eldayem, Mohammed Alqarni, Gaber El-Saber Batiha, Ahmad J Obaidullah, Mohamed Awad Shahien, Eman El-Ahwany, Noha A Amin, Mohamed Ali Etman, Mohamed MY Kaddah, Eslam E Abd El-Fattah (2022). Empagliflozin adjunct with metformin for the inhibition of hepatocellular carcinoma progression: Emerging approach for new application. Biomedicine Pharmacotherapy,145: 112455.

El Amrousy D, Elashry H, Salamah A, Maher S, Abd-Elsalam SM, Hasan S (2022). Adherence to the Mediterranean Diet Improved Clinical Scores and Inflammatory Markers in Children with Active Inflammatory Bowel Disease: A Randomized Trial. Journal of inflammation research, 15: 2075–2086.

Kamel M, Maher S, El-Baz H, Salah F, Sayyouh O, Demerdash Z (2022). Non-Invasive Detection of SARS-CoV-2 Antigen in Saliva versus Nasopharyngeal Swabs Using Nanobodies Conjugated Gold Nanoparticles. Tropical Medicine and Infectious Disease, 7(6):102.

Attia Atta, Shimaa Atta, Soad Nasr, Samar Mounir (2022): current perspective on veterinary drug and chemical residues food of animal origin. Environmental science and pollution research. Doi.org/10.1007/s11356-021-18239-y.

El-Maadawy WH, Hassan M, Abdou RM, El-Dine RS, Aboushousha T, El-Tanbouly ND, El-Sayed AM (2022). 6-Paradol alleviates Diclofenac-induced acute kidney injury via autophagy enhancement-mediated by AMPK/AKT/mTOR and NLRP3 inflammasome pathways. Environmental Toxicolology and Pharmacology, 91 (103817): 1-10.

Hassan M, Nasr SM, Elzallat M (2022). Effect of CD133 polymorphisms on the risk of developing liver cirrhosis and hepatocellular carcinoma induced by viral hepatitis. Virus Research, 312 (198714): 1-7.

El-Maadawy WH, Hassan M, Hafiz E, Badawy MH, Eldahshan S, AbuSeada A, El-Shazly MAM, Ghareeb MA (2022). Co-treatment with Esculin and erythropoietin protects against renal ischemia-reperfusion injury via P2X7 receptor inhibition and PI3K/Akt activation. Scientific Reports, 12(6239): 1-12.

Mahmoud E Youssef, Abd El-Fattah, E Eslam, Amir M Abdelhamid, Hanan Eissa, Eman El-Ahwany, Noha A Amin, Helal F Hetta, Mohamed H Mahmoud, Gaber El-Saber Batiha, Naglaa Gobba, Ahmed Gaafar Ahmed Gaafar, Sameh Saber (2021). Interference with the AMPKα/mTOR/NLRP3 Signaling and the IL-23/IL-17 Axis Effectively Protects against the Dextran Sulfate Sodium Intoxication in Rats: A New Paradigm in Empagliflozin and Metformin Reprofiling for the Management of Ulcerative Colitis. Frontiers in Pharmacology, 1: 2027-2038.

Sameh Saber, Mohamed Nasr, Ahmed S Saad, Ahmed AE Mourad, Naglaa A Gobba, Ahmed Shata, Abdel-Moneim Hafez, Ramy N Elsergany, Heba I Elagamy, Eman El-Ahwany, Noha A Amin, Samuel Girgis, Yaser HA Elewa, Mohamed H Mahmoud, Gaber El-Saber Batiha, Magdy Abou El-Rous, Islam Kamal, Mohamed MY Kaddah, Ahmed E Khodir (2021). Albendazole-loaded cubosomes interrupt the ERK1/2-HIF-1α-p300/CREB axis in mice intoxicated with diethylnitrosamine: A new paradigm in drug repurposing for the inhibition of hepatocellular carcinoma progression. Biomedicine Pharmacotherapy, 142, 112029.

El Amrousy D, El Ashry H, Maher S, Ganna S, Hasan S (2021). Pulmonary function test abnormalities in children and adolescents with non-alcoholic fatty liver disease. European journal of pediatrics, 180(6), 1693–1699.

Shimaa Atta, Fayed Megahed, Amina Ibrahim (2021): In-vitro anticancer and antioxidant activities of Eremina desertorum (Forsskal, 1775) snail mucin. Asian Pacific Journal of Cancer Prevention, 22:  3467-3474.

Shimaa Atta, Nabila E Kramani, Sara Mohamed, Menna Mohamed, Sara Hassan, Reem Hesham, Aya Mohamed, Eman Abdel Halim, Yasmeen Mohamed, Eman El Ahwany (2021): MicroRNA-199: A Potential Therapeutic Tool for Hepatocellular Carcinoma in an Experimental Model. Asian Pacific Journal of cancer prevention, 22:2771-2779.

Hoda Elsayed Mohammed, Sama Mohammed Attiyah, Shimaa Attia Atta (2021): Comparative Study on the Pro-Inflammatory Activity of Turmeric (Curcuma longa) and flaxseed (Linumusitatissimum). Annals of R.S.C.B., 25 (4): 6329 – 6335.

Abdou RM, El-Maadawy WH, Hassan M, El-Dine RS, Aboushousha T, El-Tanbouly ND, El-Sayed AM (2021). Nephroprotective activity of Aframomum melegueta seeds extract against diclofenac-induced acute kidney injury: A mechanistic study. Journal of Ethnopharmacology, 273: 113939.

EL-Ghannam M, Abdelrahman Y, Abu-Taleb H, Hassan M, Hassanien M, EL-Talkawy MD (2021). Validation of Circom comorbidity score in critically-ill cirrhotic patients. Clinical Epidemiology and Global Health, 11: 100728.

Hassan M, Aboushousha T, El-Ahwany E, Khalil HK, Montasser AY, Abu-Taleb H, El-Talkawy MD, Zoheiry M (2021). Impact of E-cadherin and its transcription regulators on assessing epithelial-mesenchymal transition in chronic HCV infection. Minerva Gastroenterology, 67(2): 175-182.

Khalil HMA, Eliwa HA, El-Shiekh RA, Al-Mokaddem AK, Hassan M, Tawfek AM, El-Maadawy WH (2021). Ashwagandha (Withania somnifera) root extract attenuates hepatic and cognitive deficits in thioacetamide-induced rat model of hepatic encephalopathy via induction of Nrf2/HO-1 and mitigation of NF-κB/MAPK signaling pathways. Journal of Ethnopharmacology, 277: 114141.

Elbaz H, Demerdash Z, Elzallat M, Mahmoud FS, Hassan S, Hassan M, Taha T, Rasheed DM, Gaafar T (2021). Passaging of CB-MSCs at high and low cell densities: impact on telomere length and differentiation potentials. Gazzetta Medica Italiana Archivio per le Scienze Mediche, 180(10): 530-538.

Sokary R, Abu el-naga MN, Bekhit M, Atta S. (2021). A potential antibiofilm, antimicrobial and anticancer activities of chitosan capped gold nanoparticles prepared by γ–irradiation. Materials Technology, 1-10.

Atta S, Kamel M, Mansour W, Hussein T, Maher K, Elrefaiy MA. (2021). Ascitic Fluid Cytokines in Chronic Liver Disease: A Possible Prognostic Tool. Digestive Diseases, DOI: 10.1159/000514356.

Saber S, Abd El-Fattah EE, Yahya G, Gobba NA, Maghmomeh AO, Khodir AE, Mourad AAE, Saad AS, Mohammed HG, Nouh NA, Shata A, Amin NA, Abou El-Rous M, Girgis S, El-Ahwany E, Khalaf EM, El-Kott AF, El-Baz AM (2021). A Novel Combination Therapy Using Rosuvastatin and Lactobacillus Combats Dextran Sodium Sulfate-Induced Colitis in High-Fat Diet-Fed Rats by Targeting the TXNIP/NLRP3 Interaction and Influencing Gut Microbiome Composition. Pharmaceuticals. 14(4):341

Mansour W, Kamel M, Elzayat E, Atta S, Mahmood D, Abd El Fattah El Sayed H, Hussein T, Saber S (2021). Therapeutic Role of Bone Marrow-Derived Mesenchymal Stem Cells in Controlling Prognosis of Hepatocellular Carcinoma in a Murine Model. Experimental and Clinical Transplantation: Official Journal of the Middle East Society for Organ Transplantation.

Badr AM, El-Ahwany E, Goda L, Nagy F, Helal N, El Deeb S. (2021). MicroRNA-26a systemic administration attenuates tumor formation in hepatocellular carcinoma mouse model. Pakistan Journal of Pharmaceutical Sciences, 34(3).

Nabi AA, Atta SA, El-Ahwany E, Elzayat E, Saleh H (2021). Taurine Upregulates miRNA-122-5p Expression and Suppresses the Metabolizing Enzymes of Glycolytic Pathway in Hepatocellular Carcinoma. Molecular Biology Reports, 1-11.

Shawki E, El-Beih NM, El-Hussieny EA, EL-Ahwany E, Hassan M, Zoheiry M (2021). Effects of free and nanoparticulate curcumin on chemically-induced liver carcinoma in animal model. Archives of Medical Science, 17 (1): 218-227.

El Baz H, Demerdash Z, Kamel M, Hammam O,  Abdelhady  D, Mahmoud S, Salah F, Hassan S, Atta S,  Riad N,  Gaafar T (2020). Induction of Hepatic Regeneration in an Experimental Model Using Hepatocyte-Differentiated Mesenchymal Stem Cells. Cellular Reprogramming, 22: 1-13.

El-Razek SEA, El-Gamasy SM, Hassan M, Abdel-Aziz MS, Nasr SM (2020). Transition metal complexes of a multidentate Schiff base ligand containing guanidine moiety: Synthesis, characterization, anti-cancer effect, and anti-microbial activity. Journal of Molecular Structure, 1203: 127381.

Hassan M, Nasr SM, Abd-El Razek SE, Abdel-Aziz MS, El-Gamasy SM (2020). New superior bioactive metal complexes of ligand with N, O donor atoms bearing sulfadiazine moiety: physicochemical study and thermal behavior for chemotherapeutic application. Arabian Journal of Chemistry 13(10): 7324-7337.

Demerdash Z, El Baz H, Ali N, Mahmoud F, Mohamed S, Khalifa R, Hassan M, Shawky S (2020). Cloning of Human Cord Blood-Mesenchymal Stem Cells for Isolation of Enriched Cell Population of Higher Proliferation and Differentiation Potential. Molecular Biology Reports 47: 3963-3972.

Fawzy Roshdy, Mohamed MS Farag, Eman El-Ahwany, Ola Mahmode, Adel A Mousa, Mohamed El Talkawy, Faiza Essawy (2020). Long non-coding RNA HOTAIR and HOTTIP as potential biomarkers for hepatitis C virus genotype 4-induced hepatocellular carcinoma. Egyptian Journal of Medical Human Genetics. 21 (1): 1-13.

Gamal Ramadan, Marwa M Mohammed, Mona K Zoheiry, Nadia El-Beih (2020). Whey protein concentrate and lactoferrin alleviated anaemia, immunotoxicity, and biochemical alterations in a mouse model of early hepatocarcinogenesis. International Dairy Journal, 102: 104603

Eman El-Ahwany, Soheir S Mansy, Mona Zoheiry, Lobna Mourad, Soheir Mahmoud, Hoda Abu-Taleb, Moataz Hassanien, Marwa Hassan (2020). MicroRNA-195 vector influence on the development of gradually induced hepatocellular carcinoma in murine model.  Ultrastructural Pathology, 44 (2): 203-210.

Sameh Saber, Amal MH Ghanim, Eman El-Ahwany, Eman M Abd El-Kader (2020). Novel complementary antitumour effects of celastrol and metformin by targeting IκBκB, apoptosis and NLRP3 inflammasome activation in diethylnitrosamine-induced murine. Cancer Chemotherapy and Pharmacology, 85 (2): 331-343.

Eman GE El-Ahwany, Lobna Mourad, Mona MK Zoheiry, Hoda Abu-Taleb, Marwa Hassan, Raafat Atta, Moataz Hassanien, Suher Zada (2020). MicroRNA-122a as a non-invasive biomarker for HCV genotype 4-related hepatocellular carcinoma in Egyptian patients. Archives of Medical Science, 15 (6): 1454-1460.

Hend Okasha, Hassan M, Tarek Aboushousha, Safia Samir (2019). Effect of Interferon-Beta (IFN-β) on Tumor suppressor and Apoptotic Markers in Hepatocellular Carcinoma Cell Line. International Journal of Research in Pharmaceutical Sciences, 10(4): 2936-2943.

Rady E El-Araby, Mahmoud A Khalifa, Mona M Zoheiry, Manal Y Zahran, Mohamed I Rady, Raafat A Ibrahim, Mohamed D El-Talkawy, Faiza M Essawy (2019). CDH1 gene as a prognostic biomarker in HCV (genotype 4) induced hepatocellular carcinoma in the Egyptian patients. Gene Reports, 16: 100452.

Marwa M Mohammed, Gamal Ramadan, Mona K Zoheiry, Nadia M El‐Beih (2019). Antihepatocarcinogenic activity of whey protein concentrate and lactoferrin in diethylnitrosamine‐treated male albino mice. Environmental toxicology, 34 (9): 1025-1033.

Mahmoud FS, Demerdash Z, Elmotawakel S, Hassan S, Hendawy M, Atta S, Shawky S, Alkhateeb E, Hassenein H (2019). Serum low replacement medium versus serum rich replacement medium for production of Anti-Schistosoma Monoclonal antibody: A comparative study. Clinical Epidemiology and Global Health, 8(2): 423-427.

Kamel M, Salah F, Demerdash Z, Maher S, Atta S, Badr A, Afifi A, El-Baz H (2019). Development of a new lateral-flow imunochromatographic strip using colloidal gold and mesoporous silica nanoparticles for rapid diagnosis of active Schistosomiasis. Asian Pacific Journal of Tropical  Biomedicine, 9: 315-322.

Rady E El-Araby, Mahmoud A Khalifa, Mona M Zoheiry, Manal Y Zahran, Mohamed I Rady, Raafat A Ibrahim, Mohamed D El-Talkawy, Faiza M Essawy (2019). The interaction between microRNA-152 and DNA methyltransferase-1 as an epigenetic prognostic biomarker in HCV-induced liver cirrhosis and HCC patients. Cancer gene therapy, 1: 1-12.

Ghanem LY, Mansour IM, Abulata N, Akl MM, Demerdash ZA, El Baz HG, Mahmoud SS, Mohamed SH, Mahmoud FS, Hassan ASM (2019). Liver macrophage depletion ameliorates the effect of mesenchymal stem cell transplantation in a murine model of injured liver. Scientific Reports, 9(1): 35.

El-Lakkany NM, El-Maadawy WH, Seif El-Din SH, Saleh S, Safar MM, Ezzat SM, Mohamed SH, Botros SS, Demerdash Z, Hammam OA (2019). Antifibrotic effects of gallic acid on hepatic stellate cells: In vitro and in vivo mechanistic study. Journal of Traditional and Complementary Medicine, 9(1): 45-53.

Sameh Saber, Rania M Khalil, Walied S Abdo, Doaa Nassif, Eman El-Ahwany (2019). Olmesartan ameliorates chemically-induced ulcerative colitis in rats via modulating NFκB and Nrf-2/HO-1 signaling crosstalk. Toxicology and applied pharmacology, 364: 120-132.

El Baz H, Demerdash Z, Kamel M, Atta S, Salah F, Hassan S, Hammam O, Khalil H, Bayuomi A (2019). Potentials of differentiated human cord blood derived unrestricted somatic stem cells in treatment of liver cirrhosis. Experimental & Clinical Transplantation, 17: 251-258.

Attia H. Atta, Samar M. Mouneir, Soad M. Nasr, Doaa Sedky, Amany M. Mohamed, Shimaa A Atta, Hassan Mohamed Desouky (2019). Phytochemical studies and anti-ulcerative colitis effect of Moringa oleifera seeds and Egyptian propolis methanol extracts in a rat model. Asian Pacific Journal of Tropical Biomedicine; 9(3): 98-108.

Heba KM Khalil, Mona Zoheiry, Nora N Kamel, Tarek Aboushousha, Eman El-Ahwany, Hoda A Taleb, Mohamed D El-Talkawi, Ali Abdel Rehim, Noha M Khalil, Marwa Hassan, Ahmed Y Montasser (2018). Hepatocellular expression of epithelial–mesenchymal transition markers in a sample of hepatitis C virus-infected Egyptian patients. Egyptian Journal of Pathology, 38 (2): 234-240.

Saber S, Mahmoud AAA, Goda R, Helal NS, El-Ahwany E, Abdelghany RH (2018). Perindopril, fosinopril and losartan inhibited the progression of diethylnitrosamine-induced hepatocellular carcinoma in mice via the inactivation of nuclear transcription factor kappa-B. Toxicology Letters, May 31, 295: 32-40.

Amanda Abdel-Al, Eman El-Ahwany, Mona Zoheiry, Marwa Hassan, Amged Ouf, Hoda Abu-Taleb, Ali Abdel Rahim, Mohamed Darwish El-Talkawy, Suher Zada (2018). miRNA-221 and miRNA-222 are promising biomarkers for progression of liver fibrosis in HCV Egyptian patients. Virus research, 253: 135-139.

Malak Nabil Amin, Ashraf Okba, Abeer Abdel Hamid Eissa, Mohamed Asharf Ibrahim, Mona Kamel Zoheiry, Abu Taleb, Hoda Mohamed Aly (2018). Th17 and tregulatory cells in type2 diabetic nephropathy.  Journal of the Egyptian Society of Parasitology, 48(3): 605-613.

Sameh Saber, Amr Mahmoud, Noha Helal, Eman El-Ahwany, Rasha Abdelghany (2018). Liver protective effects of renin-angiotensin system inhibition have no survival benefits in hepatocellular carcinoma induced by repetitive Administration of Diethylnitrosamine. Macedonian journal of medical sciences, 6(6): 955.

Lobna Mourad, Eman El-Ahwany, Mona Zoheiry, Hoda Abu-Taleb, Marwa Hassan, Amged Ouf, Ali Abdel Rahim, Moataz Hassanien, Suher Zada (2018). Expression analysis of liver-specific circulating microRNAs in HCV-induced hepatocellular carcinoma in Egyptian patients. Cancer biology & therapy, 19 (5):  400-406.

Sameh Saber, Amr AA Mahmoud, Noha S Helal, Eman El-Ahwany, Rasha H Abdelghany (2018). Renin–angiotensin system inhibition ameliorates CCl4-induced liver fibrosis in mice through the inactivation of nuclear transcription factor kappa B. Canadian journal of physiology and pharmacology, 96 (6): 569-576.

Kamel MM, El-Baz HG, Demerdash Z, Hassan S, Salah F, Mansour WA, Hammam O, Atta S, Bayoumi A, Hassan M, Mahmoud S (2018). Cord blood-derived mesenchymal stem cells with hepatogenic differentiation potential ameliorate chronic liver affection in experimental models. Advances in Clinical and Experimental Medicine 27(10):1329-1339.

Soheir S Mansy, Eman El-Ahwany, Soheir Mahmoud, Sara Hassan, Mohammed I Seleem, Amr Abdelaal, Ahmed H Helmy, Mona K Zoheiry, Ahmed S AbdelFattah, Moataz H Hassanein (2017). Potential ultrastructure predicting factors for hepatocellular carcinoma in HCV infected patients. Ultrastructural pathology, 41 (3): 206-226.

S Saber, AA Mahmoud, NS Helal, E El-Ahwany, RH Abdelghany (2017). Losartan, an angiotensin-II type 1 receptor blocker, attenuates CCl4-induced liver fibrosis with a positive impact on survival in mice. World J Pharm Pharm Sci., 5 (12): 121-126.

Khalifa RH, Labib DA, Kamel MA, Shahin RMH, Bahgat DMR, Riad NM, El Khateeb E, El-deeb AM, Hassan M (2017).  Role of ApoB-516C/T promoter gene polymorphism in the risk of Hepatitis C Virus infection in Egyptian patients and in gender susceptibility. Journal of Medical Virology, 89(9): 1584-1589.

El Baz H, Demerdash Z, Kamel M, Atta S, Salah F, Hassan S, Hammam O, Khalil H, Meshaal S, Raafat I (2017). Transplant of Hepatocytes, Undifferentiated Mesenchymal Stem Cells, and In Vitro Hepatocyte-Differentiated Mesenchymal Stem Cells in a Chronic Liver Failure Experimental Model: A Comparative Study. Experimental & Clinical Transplantation, 16: 81-89.

Youssef MM., Tolba M., Badawy NN, Andrew W. Liu, El-Ahwany E, Khalifa AE, Zada S, Abdel-Naim AB (2016). Novel combination of sorafenib and biochanin-A synergistically enhances the anti-proliferative and pro-apoptotic effects on hepatocellular carcinoma cells. Scientific Reports, 6: 30717.

Abdel-Lateef E, Hammam O, Mahmoud FS, Atta SA, El-SayedMM, Hassenein HI (2016). Induction of apoptosis in HepG2 by Vitexagnus-castus L. leaves extracts and identification of their active chemical constituents by LC-ESI-MS. Asian Pacific Journal of Tropical Disease, 6(7): 539-548.

Abdel-Lateef E, Mahmoud F, Hammam O, El-Ahwany E, El-Wakil E, Kandil S, Abu-Taleb H., El-Sayed M, Hassenein H (2016). Bioactive chemical constituents of Curcuma longa L. rhizomes extract inhibit the growth of human hepatoma cell line (HepG2).  Acta Pharmaceutical, 66: 1-13.

El-Ahwany E, Nagy F, Zoheiry M, Shemis M, Nosseir M, Taleb HA, El Ghannam M, Atta R, Zada S (2016). Circulating miRNAs as predictor markers for activation of hepatic stellate cells and progression of HCV-induced liver fibrosis. Electron Physician, 8(1): 1804-1810.

El-Hefnawi M, TaeKyuKim, Kamar M, SaehongMin, Hassan N, El-Ahwany E, Heeyoung Kim, Zada S, Amer M, Marc P. Windisch (2016). In Silico Design and Experimental Validation of siRNAs Targeting Conserved Regions of Multiple Hepatitis C Virus Genotypes. PLOS ONE, 11(7): e015921.

Zoheiry M, El-Ahwany E (2015). Role of epithelial mesenchymal transition in hepatic fibrogenesis. Journal of the Egyptian Society of Parasitology, 45(2): 357 -364. 

Abdel-Ghany R, Rabia I, El-Ahwany E, Saber S, Gamal R, Nagy F, Mahmoud O, Hamad RS, Barakat W (2015). Blockade of pge2, pgd2 receptors confers protection against prepatent Schistosomiasis mansoni in mice. Journal of the Egyptian Society of Parasitology, 45(3): 511-520.

Ahwany EG, Nagy FM, Zoheiry MK, El Ghannam MT, El Khashab MN, Ahmadi W, El-Refaiy M, Zada SK, Shaker ZA (2015). Role of T regulatory cells in chronic HCV infected Egyptian patients and their impact on the response to pegylated interferon therapy. Journal of the Egyptian Society of Parasitology, 45(2): 345-356.

Maher k, El-Baz H, Demerdash Z, Shawky S, Hassan M, Mohamed S, Mahmoud F, Khalil M, Hammam O,  Taha T (2015). Long term culture of CB-MSCs deteriorates their stemness nature through a series of biomolecular changes. Global Journal of Medicine and Medical Sciences, 3(1):097-107.

Ghinolfi D, El Baz HG, Borgonovi E, Radwan A, Laurence O, Sayed HA, De Simone P, Abdelwadoud M, Stefani A, Botros SS, Filipponi F (2014). A model for Southern Mediterranean research institute self-assessment: a SWOT analysis-based approach to promote capacity building at Theodor Bilharz Research Institute in Cairo (Egypt). Arab Journal of Gastroenterology, 15(3-4): 92-97.

Amer M, Elhefnawi M, El-Ahwany E, Awad AF, Gawad NA, Zada S, Tawab FM (2014). Hsa-miR-195 targets PCMT1 in hepatocellular carcinoma that increases tumor life span. Tumour Biology, 35(11): 11301-11309.

Demerdash Z, Mohamed S, Hendawy M, Rabia I, Attia M, Shaker Z, Diab TM (2013). Monoclonal antibody-based dipstick assay: A reliable field applicable technique for diagnosis of Schistosoma mansoni infection using human serum and urine samples. Korean Journal of Parasitology, 51(1): 93-98.

El-Ahwany E, Bauiomy IR, Nagy F, Zalat R, Mahmoud O, Zada S (2012). T regulatory cell responses to immunization with a soluble egg antigen in Schistosoma mansoni-infected mice. Korean Journal of Parasitology, 50(1): 29-35.

El-Ahwany E, Rabia I, Nagy F, Zoheiry M, Diab T, Zada S (2012). Protective role of purified cysteine proteinases against Fasciola gigantica infection in experimental animals. Korean Journal of Parasitology, 50(1): 45-51.

Demerdash Z., Diab T, Aly I R, Mohamed S, Mahmoud F, Zoheiry  M, Mansour W, Mohy E, Attia M, El-Bassiouny A (2011). Diagnostic efficacy of monoclonal antibody-based sandwich enzyme linked immunosorbent assay (ELISA) for detection of Fasciola gigantica excretory/secretory antigens in both serum and stool. Parasite Vectors, 4: 176.

Elkhafif N, El Baz H, Hammam O, Hassan S, Salah F, Mansour W, Mansy S, Yehia H, Zaki A, Magdy R (2011). CD133(+) human umbilical cord blood stem cells enhance angiogenesis in experimental chronic hepatic fibrosis. Acta Pathologica, Microbiologica, et Immunologica Scandinavica, 119(1): 66-75.

Hassanein H, El-Ahwany E, Salah F, Hamaman O, Refai L, Hamed M (2011).  Extracts of five medicinal herbs induced cytotoxicity in both hepatoma and myeloma cell lines. The Journal of Cancer Science and Therapy, 3: 10-17.

Rabia I, El-Ahwany E, El-Komy W, Nagy F (2010). Immunomodulation of hepatic morbidity in murine schistosomiasis mansoni using fatty acid binding protein. Journal of American science, 6 (7): 170-176.

Zakaria S, Youssef M, Moussa M, Akl M, El-Ahwany E, El-Raziky M, Mostafa  O, Helmy AH, El-Hindawi A (2010). Value of alpha-smooth muscle actin and glial fibrillary acidic protein in predicting early hepatic fibrosis in chronic hepatitis C virus infection. Archives of Medical Sciences, 6(3): 356-365.