Electron Microscope Department

  • Vision
  • Mission
  • Head of the Department
  • Staff Members
  • Research field
  • Achievements
  • Techniques
  • Structure
  • Training
  • Services Scope
  • Medical services
  • Projects
  • Publications
Our vision is to be a referred center in the field of Electron Microscopy for medical research, diagnosis and training at the level of the country and the regional area.
Our mission is to deliver cutting edge advanced research and to offer high standard medical diagnostic service and training facilities in our specialization.

Prof. Amira Helmy Mohamed Hussein
Email: [email protected]

 

Former Heads and Staff Members

Former Heads

Staff members

Associate members

    • Technical specialist Walid Mohamed (B.Sc. Ag.)
    • Laboratory technician Sahar Abbas
    • Laboratory technician WafaaRezk
    • Laboratory technician Shaimaa El Sayed
    • Secretary Neima Aly
    • Secretary Saeid Awaad

Our department’s research focuses on the ultrastructural studies of basic medical sciences (pathology, haematology, parasitology and microbiology) specifically in gastrointestinal, liver, and urinary system diseases caused by endemic parasitic and infectious agents. Our strategy is tailored to comply with the institute’s vision and mission and to meet the terms of the Institute research programs as detailed below:

-Morbidity Changes of Endemic Diseases

The study of ultrastructural changes occurring in tissues (liver, urinary and gastrointestinal tracts) and blood secondary to:

  • Endemic Parasitic infection  
  • Endemic infectious diseases
  • tumors and  malignancies

–  Diagnosis of Endemic Diseases

  • Ultrastructural study of premalignant and malignant changes
  • Ultrastructural study of micro-organisms intracellularly and in culture
  • Ultrastructural study of endemic parasites to explore the effect of anti-parasitic innovated drugs and the immunological changes due to host immune response

 – Management of Endemic Diseases

  • Evaluating the efficacy and toxicity of currently used and newly discovered drugs on the liver, kidney, bone marrow and other important systems in experimental models
  • Ultrastructural assessment of pathological changes of therapeutic regimens on human body organs
  • Stem cells as adjuvant therapy in liver disease

  – Control of Endemic Diseases:

  • Evaluation of the efficacy of newly introduced compounds (eg potential vaccines) at the ultrastructural level

Innovation

  • Innovation of an easy applicable technique for the simultaneous processing of cytology samples for light and electron microscopic examination (Mansy, 2004). This technique offered a new prospect for cytomorphological study , enhanced the quality of diagnosis and the  early detection or prediction of malignant lesion. The department adopts the application of this technique in its research on cells separated either from urine, blood or anybody fluid. Application and reproducibility of this technique in many centers was prooved also effective.

Conclusions from studies in the field of chronic hepatitis C & B, &hepatocellular carcinoma

  • A study focusing on the intracellular hepatitis C virus  morphogenesis and virus-host cell interactions in patients infected with HCV demonstrated, for the first time, that HCV has the potential to progress, assemble, and replicate into  mouse mammary tumor virus like  (retrovirus) depending on the host hepatocellular structure. The presumed involved mechanism was described depending on a proposed scenario for the potential capacity of the detected HCV particles to convert into retroviruses and a hypothesis for this turnover was demonstrated by figures. This novel perception offers important insights that can explain the vague mechanisms of HCV behavior in the infected hepatocytes.
  • Ultrastructural mitochondrial and rough endoplasmic reticulum changes in HCV-infected patients in correlation to hepatic steatosis are related to the associated intracytoplasmic fatty acids accumulation, the decreased serum cytochrome C and apolipoproteins A1 and B. 
  • Electron microscopy could help in detecting early apoptotic changes not easily visualized by immunohistochemistry.
  • Apoptosis of neutrophils detected by electron microscopy could be an important underlying factor in neutropenia associated with HCV infection. 
  • The presence of virus like particles in the mononuclear cells in peripheral blood of HBV & HCV infected patients was demonstrated and different morphological ultrastructural features were observed which may be of help in the follow up of the clinical course of the disease. 
  • The effect of Ozone on HCV infection at the ultrastructural level was studied and we found that it had no curative effect on HCV. This finding correlated with the detected increase of viral load in these patients. 
  • Morphometric ultrastructural study of the nucleus and intercellular spaces of the hepatocytes in chronic hepatitis C virus infection and hepatocellular carcinoma proved to be of use for the early detection of neoplastic behavior.
  • New observations have been reported concerning the visualization of new hepatocytes division or regenerative process that mimic splitting or clonal fragmentation that occurs in primitive creature in cases of hepatitis C virus infection with regenerative nodules. Also, new observations that may be of value or assist in predicting HCC and identifying the appropriate patient for surveillance have been reported. Moreover, the possible malignant potentiality of liver stem/progenitor cells has been highlighted in such cases.

Conclusions from studies in the field of schistosomiasis and hepatic fibrosis

  • The novel Enaminone derivative of 4-hydroxyquinolinone (BDHQ) was studied for its potential activity against murine schistosomiasis and was found to exhibit high levels of activity against the adult and juvenile stages of the parasites.
  • By studying the effect of the new emerging antischistosomalcompoundMirazid at the ultrastructural level, it proved ineffective on schistosome worms. This had great impact on the taken measures concerning production of this drug.
  • We demonstrated that Artemisia inculta extract, that possesses antischistosomal properties, didn’t change the ultrastructural morphology of the bone marrow cells of the infected host when used as an antischistosomal agent.
  • The department succeeded to highlight the importance of Octreotide and anti-TGF Beta as antifibrotic drugs and the hazardous effect of Pentoxiphylline as antifibrotic drug when used in an advanced stage of liver fibrosis. Moreover, intravascular haemostatic behavior of blood platelets was demonstrated in patients with schistosomal fibrosis.
  • Protease enzymes are known to have a crucial role in disease propagation, and inhibitors of such proteases might emerge with promising therapeutic effects in treatment of parasitic diseases. Therefore, assessment of cystine protease expression in Schistosoma mansoni- infected mice at the ultrastructural level, and the haematological role of its inhibition in control of schistosomiasis were studied.
  • We investigated the effect of immunization with soluble larval crude antigen (SLCA) on immune response, bone marrow and Schistosoma mansoni worm with and without praziquantel (PZQ). We concluded that combined administration of PZQ and SLCA, when compared with PZQ alone, had improved the resistance to reinfection and had dual benefits of both treatments.

Conclusions from studies in the field of urology and urine cytology

  • The department conducted a research work that applied the innovated “Agarose Cell Block Technique” (Mansy, 2004) in the processing of urine cytology for the simultaneous examination by light and electron microscopy. The application of this technique proved to be effective in increasing the sensitivity of urine cytology for the determination of malignant cells. This work also highlighted the importance of electron microscopy as a diagnostic tool in controversial cases and in predicting tumor recurrence in patients under postoperative regimen of treatment as chemotherapy or immunotherapy.
  • The department applies specific parameters, deduced from this research work, for the prediction of cancer bladder and for the differentiation between dysplastic cells and low grade malignant urothelial cells.
  • The study of the differential expression of EGFR family members, TGF α, and Herregulin in normal urothelium, inflammatory, preneoplastic and neoplastic bladder lesions proved to be useful in determining their diagnostic and prognostic significance in these lesions.
  • The ultrastructural study of lymphocytes in patients with bladder carcinoma detected associated ultrastructural morphological changes in the blood lymphocytes of these patients. This may be of value in understanding the pathogenesis of this lesion.

Conclusions from studies in the field of microbiology

  • Study of the effect of different antibiotics on the ultrastructural morphology of some resistant bacterial strains and its correlation to the mechanism of action of the antibiotic used.
  • Study of different types of biofilms formed on urinary catheters and their relation to UTI and urosepsis.
  • The relation between the type of bacteria and the morphology of the formed biofilm as detected by electron microscopy could have an important inference in the mechanism of biliary stent occlusion and patency duration.
  • Assessment of time of removal of endotracheal tube through grading of biofilms and their colonization with multidrug resistant bacteria to prevent ventilator associated pneumonia.

Conclusions from studies in stem cell research

  • A research plan was designed to study the potential role of transplanted stem cells as adjuvant therapy in chronic liver diseases using murine schistosomiasis as an experimental model. We aimed at evaluating the fate of the stem cells, transplanted into the schistosome-infected mice and to study their contribution to the hepatic regeneration and apoptosis. This experimental model is essential for a deep understanding of the role and possible risks of stem cell transplantation and should be helpful in the application of these procedures in human beings. Three different sources of stem cells were transplanted:
  • Sex mismatched mice bone marrow cells were transplanted in schistosome- infected mice. These cells were detected in the livers of the sacrificed mice by anti Y chromosome, FISH technique and their transdifferentiation to hepatic lineage was proved by immunohistochemistry techniques using anti cytokeratin 19 and 20 antibodies. Ultrastructural study of the transplanted livers showed the contribution of the transplanted cells to the regeneration of the infected livers as evidenced by an increase in the number of small juvenile hepatic cells in comparison to control mice (Results are published)
  •  Fresh human umbilical cord blood cells without culture were transplanted in schisosome-infected mice (EM) and also in carbon tetrachloride poisoned mice.
  •  Isolated CD 133 +ve human umbilical cord blood stem cells were cultured and expanded to provide a large number of stem cells. After a period of 2 weeks in culture, cells were transplanted into mice. Ultrastructural and immunohistochemical examinations of the hepatic tissue of these different mice study groups were done and the results showed that the transplanted cells mainly contributed to angiogenesis rather than to hepatocytic regeneration (Results are published).
  • Another stem cell study focused on the determination of the degree of mobilization of bone marrow derived hepatic stem cells (BMHSC) into the peripheral blood and liver tissue of patients with chronic hepatic affection and correlating it with various grades of liver damage. Various degrees of severity in CLD neither evoked the mobilization of BMHSC into the circulation nor triggered their homing into liver tissue, thus excluding extrahepatic stem cell-mediated repair. The recovery process seems to be dependent on proliferating endogenous liver progenitors (OV6+ cells).
  • Another stem cell study focused on the Modulatory effect of stem cell microenvironment of injured liver tissue on transplanted MSCs. Superparamagnetic iron oxide (SPIO) – labeled MSCs were injected into mice in which liver fibrosis by carbon tetrachloride CCl4 was induced. And to identify the role of macrophages as one cellular component of the niche, selective hepatic macrophage-depleted animal model subgroup was used. Transplanted labeled stem cells are then traced for their homing into their niche and were studied for their differentiation and these activities were correlated with various levels of soluble factors released in the stem cell niche at early and late stages of liver fibrosis.
  •  Results demonstrated that MSC transplantation is a beneficial therapeutic strategy in chronic liver diseases on behalf of its anti-inflammmatory, antifibrogenic and transdifferentiation potentials. However, macrophages (as one cellular component in the stem cell niche) were found to interfere with these functions necessitating.
  1.  Conventional techniques for electron microscopy (transmission and scanning electron microscopy)
  2.  Cryo-electron microscopy
  3.  Immunohistochemistry of tissue sections and cells:
            –  Immunofluorescence
            –  Immunoperoxidase
  4.  Immunoelectron labeling
  5.  Innovated agarose cell block technique for processing of cytology samples (innovated by Mansy, 2004)
  6.  Negative staining for microorganisms
  7.  Nanoparticles examination

The department includes

      • A sample processing room
      • A semithin and ultrathin microtomy room
      • A cryo-ultramicrotomy room
      • A transmission electron microscope (TEM) room
      • A scanning electron microscope (SEM) room
      • A photo development dark room.

 Equipment and facilities

      • Philips Transmission Electron Microscope 208-S with a cell image analysis set and a video system
      • FEI Scanning Electron Microscopy
      • Ultramicrotome: Leica ultracut .
      • Ultramicrotome: LKB Broma 8800 Ultratome III
      • Cryo- ultramicrotome
      • Knifemaker: Leica EM KMR2
      • Knifemaker: LKB 7800
      • Equipment for developing photos
          • Enlarger: Beseler 45V-XL
          • Drier: DEVAPPA 3200
          • Developing set
      • Midi MACS for cell separation

 Other facilities: Incubators, water bath, cooling centrifuge, centrifuge Rotofix, refrigerator, deep freezer -80o C and Computers.

The electron microscopy research department is unique in having an assembly of highly qualified specialized researchers in pathology, hematology and microbiology.

The department organizes bi-annual training courses, concerning the techniques used in electron microscopy and its applications in research and diagnosis for university graduates and post graduate candidates. Special training courses are organized for technicians working in this field.

The department gives the opportunity of two scholarships every two years for the fulfillment of M.Sc. in the field of ultrastructural pathology, hematology and microbiology.

Medical services
Ultrastructural pathological diagnosis of different types of human specimens (tissue, blood, body fluids and smears) especially specimens from liver, kidney, urinary bladder and muscle sent from TBRI hospital or referred from other hospitals or private clinics.

Scientific consultations
The department performs scientific consultations to internal and external researchers concerning specimen manipulation, processing and diagnosis.

Ultrastructural examinations of human biopsies, smears and aspirations are performed upon clinician’s request and not on a routine basis.

Role of hepatic stellate cell, myofibroblast and fibroblast in hepatic fibrosis on top of hepatitis C virus infection; an ultrastructural and immunohistochemical study (Prof. SoheirMansy, 2010)

Electron microscopic and time kill study of the effect of linezolid alone and combined with other antibiotics on methicillin-resistant staphylococcus (MRSA) (Prof. Hoda Yehia, 2011).

Modulatory effect of stem cell microenvironment of injured liver tissue on extrinsically administered stem cells (Prof. Lobna Ghanim, 2012).

Biofilm on endotracheal tubes ( Dr. Magda Azmy, 2013).

Impact of occult hepatitis B and C viral infection on the development of hepatocellular carcinoma (Prof. Abeya Lotfy, 2013).

Ultrastructural study of mitochondrial changes in relation to carbamoyl phosphate synthase 1 in different stages of hepatitis C virus infection (Prof. SoheirMansy, 2013).

Experimental transplantation of hepatocytes (Prof. Soheir Mansy, 2013).

Occult hepatitis C virus infection in patients with malignant lymphoproliferative disorders (Prof. Abeya Lotfy, 2014).

Ultrastructural Study of Mitochondrial Changes in Relation to Carbamoyl Phosphate Synthetase 1 in Different Stages of Hepatitis C Virus Infection (Prof. Soheir Mansy, 2015).

Ultrastrutural study of hepatocytes and hepatic progenitor cells in patients with chronic hepatitis C virus infection and hepatocellular carcinoma (Prof. Soheir Mansy, 2015).

Experimental transplantation of hepatocytes (Prof. Nagui Edward, 2015), Prof. SoheirMansy Co-Principal Investigator.

Publications in the Last Ten Years

Reinier J de Vries, Shannon N Tessier, Peony D Banik, Sonal Nagpal, Stephanie EJ Cronin, Sinan Ozer, Ehab OA Hafiz, Thomas M van Gulik, Martin L Yarmush, James F Markmann, Mehmet Toner, Heidi Yeh, Korkut Uygun (2020): Subzero non-frozen preservation of human livers in the supercooled state. Nature Protocols, 1-17.

Siavash Raigani, Negin Karimian, Viola Huang, Anna M Zhang, Irene Beijert, Sharon Geerts, Sonal Nagpal, Ehab OA Hafiz, Fermin M Fontan, Mohamed M Aburawi, Paria Mahboub, James F Markmann, Robert J Porte, Korkut Uygun,

Martin Yarmush, Heidi Yeh (2020): Metabolic and lipidomic profiling of steatotic human livers during ex situ normothermic machine perfusion guides resuscitation strategies. PloS One, 15 (1).

Reinier J de Vries, Casie A Pendexter, Stephanie EJ Cronin, Beatriz Marques, Ehab OA Hafiz, Alona Muzikansky, Thomas M van Gulik, James F Markmann, Shannon L Stott, Heidi Yeh, Mehmet Toner, Korkut Uygun, Shannon N Tessier (2020): Cell release during perfusion reflects cold ischemic injury in rat livers. Scientific Reports, 10 (1), 1-14.

Viola Huang, Negin Karimian, Danielle Detelich, Siavash Raigani, Sharon Geerts, Irene Beijert, Fermin M Fontan, Mohamed M Aburawi, Sinan Ozer, Peony Banik, Florence Lin, Murat Karabacak, Ehab OA Hafiz, Robert J Porte, Korkut Uygun, James F Markmann, Heidi Yeh (2020): Split-Liver Ex Situ Machine Perfusion: A Novel Technique for Studying Organ Preservation and Therapeutic Interventions. Journal of Clinical Medicine, 9 (1), 269.

Reinier De Vries, Shannon Tessier, Peony Banik, Sonal Nagpal, Stephanie Cronin, Sinan Ozer, Ehab Hafiz, Thomas Van Gulik, Martin Yarmush, James Markmann, Mehmet Toner, Heidi Yeh, Korkut Uygun (2019): Super cooling of Human Livers to Extend the Preservation Time for Transplantation. Conference: Abstract/Cryobiology 91, 156.

M Aburawi, S Mert, E Hafiz, P Mahboub, H Yeh, K Uygun, J Markmann (2019): Evaluating Liver Regeneration during Normothermic Machine Perfusion in Hepatectomized Rat Liver Model.Conference: Abstract/ American Journal of Transplantation, 19, 342-343      

E Hafiz, B Bulutoglu, M Jaramillo, Y Chen, SS Kelangi, BE Uygun (2019): Scaffolds for liver regeneration. A Book: Handbook of Tissue Engineering Scaffolds: Volume Two, 741-764.

Negin Karimian, Siavash Raigani, Viola Huang, Sonal Nagpal, Ehab OA Hafiz, Irene Beijert, Paria Mahboub, Robert J Porte, Korkut Uygun, Martin Yarmush, Heidi Yeh (2019): Subnormothermic Machine Perfusion of Steatotic Livers Results in Increased Energy Charge at the Cost of Anti-Oxidant Capacity Compared to Normothermic Perfusion. Metabolites, 9 (11), 246.

Reinier J de Vries, Shannon N Tessier, Peony D Banik, Sonal Nagpal, Stephanie EJ Cronin, Sinan Ozer, Ehab OA Hafiz, Thomas M van Gulik, Martin L Yarmush, James F Markmann, Mehmet Toner, Heidi Yeh, Korkut Uygun (2019): Supercooling extends preservation time of human livers. Nature Biotechnology, 1-6.

El-Sheikh R.M., Mansy S.S.,  Nessim I.G., Hosni H.N.,  Hindawy A.,  Hassanein M.H., Abdel-Fattah A.S. (2019). Phosphate synthetase 1 (CPS1) as a prognostic marker in chronic hepatitis C infection. Journal of Pathology Microbiology and Immunology, 127: 93–105.

AbouSamra M.M, Basha M., Awad G.E.A., Mansy S.S. (2019).  A promising nystatin nanocapsular hydrogel as an antifungal polymeric carrier for the treatment of topical candidiasis. Journal of Drug Delivery Science and Technology, 49: 365–374.

Ghanem LY, Mansour IM, Abulata N, Akl MM, Demerdash ZA, ElBaz HG, Mahmoud SS, Mohamed SH, Mahmoud FS, Hassan ASM  (2019).Liver Macrophage Depletion Ameliorates The Effect of Mesenchymal Stem Cell Transplantationina Murine Model of Injured Liver. SciRep., 9(1):35.doi:10.1038/s41598-018-37184-4.

Fatma A. Fathy.Elmougy, Reham A. Mohamed, Mona M. Hassan, Suzan M. Elsheikh, Fatma-elzahraa M. Ahmed, Rady E. Elaraby (2019). Study of serum microRNA19a and microRNA223 as potential biomarkers for early diagnosis of hepatitis C virus-related hepatocellular carcinoma. Gene Reports, 15: 100398.

EL-Sheikh R., Mansy S., Nessim I., Hosni H, ElHindawi A., Hassanein M., Abdelfattah A (2018).  Carbamoyl phosphate synthetase 1 (CPSI) as aprognostic marker in chronic hepatitis C infection. APMIS, 127(2): 93-105.

 Ehab Hafiz, Basak Uygun, Soheir Mansy, Ali El-Hindawi, Martin. Yarmush (2018): Hepatic organoids co-populated with hepatocytes and cholangiocytes: Towards engineered liver grafts with biliary drainage. Conference: Abstract/ ULTRAPATH XIX.

Irene Beijert, Safak Mert, Viola Huang, Negin Karimian, Sharon Geerts, Ehab OA Hafiz, James F Markmann, Heidi Yeh, Robert J Porte, Korkut Uygun (2018): Endothelial Dysfunction in Steatotic Human Donor Livers: A Pilot Study of the Underlying Mechanism During Subnormothermic Machine Perfusion. Transplantation Direct, 4 (5).

 Hassan S., MansyS.S.,, Tabak S.A., AbdelFattah A.S., Abdel-Aziz A.M., Hamam O., Seleem M.I., Abdelaal A.(2018).  Immunohistochemical and electron microscopic morphometric image analysis of hepatocellular carcinoma in association of HCV infection. Ultrastructural Pathology, 42:1-11.

Beijert I, Mert S, Huang V, Karimian N, Greets S, Hafiz E, Markmann JF, Yeh H, Porte RJ, Uygun K. (2018): Endothelial Dysfunction in Steatotic Human Donor Livers: A Plot Study of the Underlying Mechanism During Subnormothermic Machine Perfusion. Transplant Direct, 4(5): e345.

Basha M., abousamraM.M,Awad G.A, Mansy S.S. (2018) .Apotential antibacterial wound dressing of cefadroxil chitosan nanoparticles in situ gel: Fabrication,in vitro optimization and in vivo evaluation international journal of pharmaceutics 544:129-140.

Mohamed S.H., Salem D, Azmy M, S. Fam N.S. (2018): Antibacterial and antibiofilm activity of cinnamaldehyde against carbapenem-resistant Acinetobacter baumannii in Egypt: Invitro study. Journal of applied pharmaceutical science Vol .8:151-156, November

Mohamed S.H., Mohamed M.S.,Khalil M.S , M. Azmy(2018)   Combination of essential oil and ciprofloxacin to inhibit /eradicate biofilms in multidrug-resistant Klebsiella pneumoniae .Journal of applied Microbiology 125 :84-95

Al‐Hashem F, Al‐Humayed S, Amin S N, Kamar SS, MansySS,Hassan  s, Lubna O. Abdel‐Salam, Abd Ellatif A, Alfaifi M, Haidara M, Al‐Ani B. (2018). Metformin inhibits mTOR–HIF‐1α axis and profibrogenicand inflammatory biomarkers in thioacetamide‐induced hepatic tissue alterations.  J Cell Physiol. 2018;1–10

Mansy S.S.El-Ahwany E.Mahmoud S., Hassan S., Seleem M.I., Abdelaal A.Helmy A.H., Zoheiry M.K., AbdelFattah A.S.Hassanein M.H. (2017). Potential ultrastructure predicting factors for hepatocellular carcinoma in HCV infected patients. Ultrastructural Pathology, 41:209-226.

Fam N, Gamal D, Azmy M., Wasfy R., AbouElFadl1 L ,Badr M, El-Damarawy M. (2017): Antimicrobial Efficacy of Doripenem Colistin Combination on Carbapenem-Resistant Acinetobacter Baumanii isolates by E-test agar Dilution and UltrastucturalMethods.TheEgyptian Journal of Medical Microbiology. 26(1).

Badawi H, El-Said M., Helmi A, Azmy M., Riad E, Beshr M and Helmy O (2016).Detection of Biological Nanoparticles and their Association with Aluminum and Barium in Serum, Urine and Renal Stone in Egyptian Patients with Urolithiasis . Ciência e TécnicaVitivinicola(31): 341-357.

Hammam OA, Elkhafif N, Attia YM, Mansour MT, Elmazar MM, Abdelsalam RM, Kenawy SA, El khatib AS. (2016). Wharton’s Jelly derived mesenchymal stem cells combined with Praziquantel  as a potential therapy for Schistosoma mansoni induced liver fibrosis. Scientific reports. 15:6:2005.

Badawi H, El-Said M., Helmi A., Hassan M., El- Ansary M, Helmy AH, Azmy M and Helmy O (2016). Role of Nanoparticles in Egyptian patients with biliary lithiasis and their association with Aluminum and Barium in serum and urine.Ciência e TécnicaVitivinicola ; 31(4): 162-177.

Azmy M, Ghanem L, Kassem F, Gamal D, Abdelkhalek A, El-Kholy E, AboELEneen  M.(2016).Scanning Electron Microscopic Biofilm Grading and Ventilator Associated Pneumonia in Relation to Duration of Intubation.The Egyptian Journal of Medical Microbiology .Vol 25(3) July 81-88.

Ismail S, Ali I, Amer N, Fahmy Z, Azmy  M and Magdy M.(2016): Susceptibility of Blastocystis hominis to Monolaurine (Lauric acid), Lactoferine (Lactobacillus acidophillus) and Metronidazole: An in Vitro and in Vivo Studies. African Journal of Pharmacy and Pharmacology Vol. (10) No.2: 14-25.

Diab M, Shemis M, El-Ghannam M, Gamal D, Azmy M, Salem D, Mansy S, Saber M.(2016). Helicobacter pylori vacA genotyping in relation to cagA status, ultrastructure of gastric mucosa and clinical outcomes in Egyptian patients. African Journal of Microbiology Research; 10: 465-472.

El-komy W, Mahmoud S, Sabry H, Mansy S, Guirguis N. (2016). Changes in parasitic load, phagocytosis and ultrastructural pattern in experimental cryptosporidiosis following combined Antox and Nitazode treatment. Journal of Educational Research and Review; 5: 038-044.

Yehia H, Said M, Azmy M, Badawy M, Mansy S, Gohar H, Madany N. (2016).Effect of linezolid alone and in combination with other antibiotics on Methicillin-Resistant Staphylococcus Aureus. J. Egypt. Soc. Parasitol  46: 57-66.

Mansy SS, Nosseir MM, Othman MM, Zoheiry MA, Guda MF, Yehia HA, Hassanein MH. (2016). Spotlight on the three main hepatic fibrogenic cells in HCV-infected patients: Multiple immunofluorescence and ultrastructure study. Ultrastructural  Pathology.40: 276-287.

Elanany M, Sherif M, Azmy M, Ahmed A. (2016). Direct Detection of Carbapenemase and ESBL producing organisms in Blood Culture. Egyptian Journal of Medical  Microbiology. 25:25- 31 .

Azmy M, Nawar N, Mohiedden  M. and Warille L. (2016). Electron Microscopic Assay of Bacterial Biofilm Formed on Indwelling Urethral Catheters. Journal of the Egyptian Society of Parasitology (JESP), 46(3): 475-484.

Hammam O, Elkhafif N, Attia Y (2015)Efficacy of Wharton’s Jelly derived mesenchymal stem cells combined with Praziquantel in Schistosoma mansoni induced liver fibrosis in mice.Conferance papers in Journal of Hepatology ,annual meeting of the EASL, Vienna, Austria

Attia YM, Hammam OA, Elkhafif N., Mansour T, Elmazar MM, Abdelsalam RM, Kenawy SA and El-khatib AS. (2015). Successful integration of transplanted mesenchymal stem cells into the livers of schistosomamansoni– infected mice. International journal of development research.5 (03):3847-3851.

Mansy S., Nosseir M., Zoheiry M., Hassanein M., Guda M., AbuTalleb H. (2014). Value of reelin for assessing hepatic fibrogenesis in a group of Egyptian HCV infected patients. Clinical and  Chemical  Laboratory  Medicine,  52 : 1319-1328.

Khalil R.M., Abd-ElBary A., Kassem M., El Ridy M.S., Abousamra M.M., Awad G.E., Mansy S. (2014). Formulation and characterization of nystatin-loaded nanostructured lipid carriers for topical delivery against cutaneous candidiasis. British journal of Pharmaceutical Research 4:491-496.

Ghanem LY, Nosseir MF, Lotefi AA, Mohamed AS, Ibrahim RA, Hassanein MH, Mansour E, Makhlouf MM, Fouad YM, EL-Khayat HR (2012). Hematopoietic stem cell mobilization into the peripheral circulation in patients with chronic liver diseases. Journal of Digestive Diseases. 13; 571–578.

Helmy A., Kishta S. and Khaled I. (2010). Interrelation between peripheral platelet count and platelet activation during and after liver surgery in pigs. Blood CoagulFiblinolysis. 21(3): 237-41.

Elkhafif N., Voss B., Hammam O., Yehia H., Mansy S., Akl M., Boehm S., Mahmoud S, El Bendary O, El Fandy G. (2010).Homing of transplanted bone marrow cells in livers of     Schistosoma mansoni infected mice. APMIS, 118: 277-287.

Elkhafif N., ElBaz H., Hamam O., Hassan S. , Salah F. , Mansour W.   , Yehia H., Magdy R., Zaki A. (2010) .CD 133 +human umbilical cord blood stem cells enhance angiogenesis in experimental chronic hepatic fibrosis. APMIS, 119: 66-75.

Mansy S., Elkhafif N.,  Abd el Fatah A. , Yehia H ,  Mostafa I. (2010) .Hepatic stellate cells and fibrogenesis in cases of HCV infection: “An ultrastructural insight”. Journal of Ultrastructural Pathology, 34: 62-67.