Hematology Department

  • Vision
  • Mission
  • Head of the Department
  • Staff Members
  • Research field
  • Achievements
  • Techniques
  • Structure
  • Training
  • Services Scope
  • Medical services
  • Projects
  • Publications
  • Honorary Prizes

  • establishing an advanced coagulation unit concerned with the clinical setting of coagulopathy, bleeding disorders and factors deficiency.
  • establishing a stem cell research lab to handle the new strategies of stem cell therapy especially with respect to differentiation into hepatocytes.
  • upgrading the cell separation unit to include novel technologies for optimum transfusion therapy and future products marketing.
  • designing a comprehensive plan for blood donation campaigns to provide abundant supply of blood groups and to transform the hematology blood bank unit into a larger one that can cover the entire vicinity of tbri.
  • provision of highly qualified laboratory services that comply with the latest who regulations for laboratory quality management system (iso15189) to raise both patient and physician satisfaction and compete for market share.
  • Improvement of health care and curative services through new technologies and advanced diagnostic services in the fields of hematology, blood banking, cellular and transfusion medicine to meet both routine and emergency needs.
  • To provide accurate laboratory results within appropriate turnaround time to meet satisfaction of care providers and patients.
  • To provide the highest standard quality blood and blood components for emergency and routine medical requirements.
  • To maintain and improvise the role of Hematology Department and Blood Bank in liver transplant procedure.
  • Organizing specific training courses for medical, paramedical and technical students.

Prof. Dr. Manal Youssef Zahran
Email: [email protected]

 

 

Former Head of Hematology  Department 

    • Prof. Sawsan A. Omran   (1978-1999).
    • Prof. Faiza M. Essawy   (1999-2005).
    • Prof. Salwa M. Toima   (2005-2010).
    • Prof. Nora E. El-Bassiouni (2011-2015).
    • Prof. Maha A. El-Mansy (2015-2018).
    • Prof. Iman A. Khaled (2018- 2020)

Staff Members

    • Prof. Dalal A. Aboul-Magd
    • Prof. Aliaa M. Amer
    • Prof. Nariman M. Zahran
    • Prof. Bothina S. Madkour
    • Prof. Faiza M. Essawy
    • Prof. Nora E. El-Bassiouni 
    • Prof. Maha A. El-Mansy
    • Prof. Nadia A. Hussein
    • Prof. Hamda H. El-Sayed
    • Prof. Iman A. Khaled
    • Prof. Manal Y. Zahran
    • Prof. Ola M. Mahmoud
    • Ass. Prof. Mona E. Madkour
    • Ass. Prof. Azza S. Hassanien
    • Ass. Prof. Iman W. Bekheet
    • Ass. Prof. Abeya F. Saleh
    • Ass. Prof. Omar M. Sabry
    • Researcher. Marwa S. Wahdan
    • Researcher. Rabab F. Yassin
    • Researcher. Noha A. Amin
    • Researcher. Ola B. Metwaly
    • Ass. Researcher. Rania M. Benyamin
    • Ass. Researcher. Emad Y. Matta
    • Ass. Researcher. Maged M. Mussallam
    • Ass. Researcher. Shahinaz M. Mourad
    • Ass. Researcher. Amira Ramadan
    • Ass. Researcher. Sara Hassan
    • Ass. Researcher. Asmaa El Saeed
    • Ass. Researcher. Mohamed Salah
    • Ass. Researcher. Khaled Twfeek
    • Resident. Noha Elshishtawy
    • Resident.  Hend Gamal
    • Resident.  Amr Mahmoud Abdel Sabour
    • Resident.  Mohamed Hossam El Din Elsharkawy 
  • Haematological disorders in chronic liver and kidney diseases.
  • Haemostatic defects.
  • Disseminated Intravascular Coagulopathy (DIC).
  • Anaemia and erythropoiesis.
  • Cell culture.
  • Flowcytometric analysis of haematological and immunological cell surface markers.
  • Gene analysis and cytogenetic studies among haemodialysis patients and those with chronic liver disease.
  • Blood banking updates.
  • Studies of platelet preparation and storage: New modalities.
  • Studies of leucocytes filtration on blood transfusion.
  • Studies of the effect of cold promoted activation during storage on the haemostatic cascade.
  • Studies of red cell changes in multi-transfused patients.
The Haematology Department research based projects, whether finalized or running; explore the haematological disorders and haemostatic abnormalities that may accompany the clinical course of chronic liver and kidney diseases, with special emphasis on pathophysiologic, aetiological, diagnostic and prognostic aspects of anaemia, coagulation abnormalities, fibrinolysis, endothelial and leucocytes changes, cytokine and chemokine profiles. The genetic polymorphism of thrombophilia, platelet ultrastructure and stem cell culture were also studied. Advanced diagnostic research in the fields of blood banking, cellular and transfusion medicine were also conducted.
  • Automated haemogram including complete blood count and blood film examination.
  • Semi-automated turbodensitometric method using magnetic beads for routine coagulation tests.
  • Automated technique using chronometric and photometric method to conduct specific tests for diagnosis of coagulation defects and monitoring anticoagulant therapy.
  • Platelet function tests using aggregometer.
  • Blood grouping tests by slide method, tube method and column agglutination technique (CAT).
  • Compatibility tests using tube method and CAT.
  • Preparation of PRBC, fresh frozen plasma, platelet concentrates and other blood components using manual and aphaeresis techniques.
  • ELISA techniques for detection of viral hepatitis markers (HBs Ag and HCV Ab) and HIV (Ag and Ab), assessment of iron profile (S. ferritin) and coagulation assay (D-dimer).
  • Chemilumenicence technique for instant determination of viral hepatitis markers (HBV and HCV), HIV, syphilis, iron profile (S. ferritin and S. iron) and coagulation assays (D-dimer).
  • Flowcytometric analysis for immunophenotypic detection of specific hematological and immunological surface markers.
  • Bone marrow aspiration and trephine biopsy.
  • Examination of blood smears for detection of haemoparasites.
  • Analysis of the acetic fluid.
  • Cell culture techniques.

The Hematology Department involves the following specialized units:

Research Laboratories
They are designed for the performance of research work, related scientific projects and theses. They comprise a cell culture unit and a stem cell unit.

Out-Patients Clinic Laboratories.
They provide the routine investigations required by both the out- and in-patients. The service runs on a 24-hour basis.

Blood Bank.
This unit is in charge of blood donation services to screen healthy donors for transfusion-transmitted viral and bacterial infections, to prepare blood and blood components (packed RBCs, fresh frozen plasma and platelet concentrates) and to provide necessary blood transfusion to emergency needs and patients undergoing surgery.

Summer training courses for one-week rotation in the field of basic clinical laboratory tests and techniques for under-graduate candidates of the Faculties of Science and Agriculture, Cairo and Ain Shams Universities, Cairo, Egypt.

Certified training courses for two months rotation in the field of advanced and novel hematology and blood banking techniques for post-graduate house-officers of the Faculty of Applied Medical Science, 6th October University, Giza, Egypt.

Specific periodical training courses for two weeks in the field of advanced hematology and clinical & chemical pathology for TBRI staff members, medical and paramedical personals.

  • The haematology out-patients’ clinic services encompass the services offered to out- and in-patients haematological diseases, especially those complaining of haematological abnormalities and haemostatic defects associated with chronic liver and kidney diseases.
  • Diagnosis and follow up of patients with coagulation defects and monitoring short and long term anticoagulant therapy using specific standard tests and INR evaluation.
  • Proper diagnostic and follow up tests needed for urologic and surgical patients are also performed. Patients with hypersplenism are thoroughly investigated and bone marrow examination is resorted to confirm the diagnosis.
  • The Blood Bank unit insures healthy donors screening for viral and bacterial markers, blood grouping and compatibility testing, preparation of blood and blood components and provision of necessary blood transfusion to emergency needs and patients undergoing surgery.
  • Haematology Lab provide 24 hours services for prediction, diagnosis and follow up of hematological abnormalities and haemostatic defects associated with chronic hepatic and renal diseases. Hematological diseases such as disorders of red and white blood cells, platelets and coagulation defects and bleeding disorders are commonly encountered.
  • Complete blood count and examination of blood films.
  • Reticulocytic count.
  • ESR.
  • Iron profile (S. iron and S. ferritin).
  • Hb electrophoresis.
  • Coagulation profile (PT, PTT and factors assay).
  • Ascetic fluid analysis.
  • Bone marrow aspiration and trephine biopsy.
  • Examination of blood smears for detection of haemoparasites.
  • Blood grouping and compatibility tests.
  • Direct and indirect Coomb’s test.


Cooperative Projects

Circulating cancer stem cells markers: Diagnostic tools in chronic/cirrhotic viral hepatitis patients. ID: 107 Diagnosis, Dr. Noha A. Amin (2018-2020), funded by TBRI.

TBRI Biobank for Liver Diseases: Research Translation and Precision Medicine in Hepatology. Dr. Noha A. Amin (2020-2023), funded by STDF, STDF Grants for Accredited Centers of Scientific Excellence (STDF-ACSE).

Epigenetic regulation of circulating cancer stem cell in HCV-induced hepatocellular carcinoma. Dr. Noha A. Amin (2020-2022), funded by STDF, Basic and Applied Research Grant (BARG), Call 6.

Polymer Nanoparticle Mediated Delivery of MicroRNA: A Novel Potential Therapeutic Tool for Hepatocellular Carcinoma in an Experimental Model. Dr. Noha A. Amin (2019-2021), funded by Science Technology Development Fund (STDF), Basic and Applied Research Grant (BARG), Call 4.

Long Non-Coding RNA: An Innovative Therapeutic Tool for Hepatocellular Carcinoma in vitro and in vivo. Dr. Noha A. Amin (2019-2021), funded by STDF, Innovative Grant, Call 6.

  • Raafat M, M Nabil, N Sherif1, A Metwaly, M Zahran ,O Sabry (2020): Evaluation of statin anti-inflammatory effect on atherosclerosis in chronic kidney disease. Medical Science, 24 (103).

  • Roshdy F, M. Farag , E. El-Ahwany , O. Mahmoud , A. Mousa , M. El Talkawy and F  Essawy (2020). Long non-coding RNA HOTAIR and HOTTIP as potential biomarkers for hepatitis C virus genotype 4-induced hepatocellular carcinoma. Egyptian Journal of Medical Human Genetics,

  • El-Bassiouni NE, Amin NA, El Amir AM, Farid AA, Madkour ME, Atta RI (2020). Differential Recruitment of Monocytes Subsets in Chronic Hepatitis C Patients.OpenAccess Macedonian Journal of Medical Sciences (in press).

  • Amin NA, El Talkawy MD, Aboushousha TS, El Ahwany EG, Zoheiry MK, El-Bassiouni NE (2019): Contribution of infiltrating monocytes to HCV-induced hepatic fibrosis and carcinogenesis: Immunohistochemical study. Abstract / Journal of Advanced Research, 16: 157–165.

  • El-Araby R, M Khalifa, M Zoheiry, M. Zahran, M. Rady, R. Ibrahim, M. El-Talkawy, F. Essawy (2019).The interaction between microRNA-152 and DNA methyltransferase-1 as an epigenetic prognostic biomarker in HCV induced liver cirrhosis and HCC Patients. Cancer Gene Therapy https://doi.org/10.1038/s41417-019-0123-9.

  • El-Araby R, M Khalifa, M Zoheiry, M Zahran, M. Rady, R. Ibrahim, M. El-Talkawy, F Essawy (2019). CDH1 gene as a prognostic biomarker in HCV (genotype 4) induced hepatocellular carcinoma in the Egyptian patients. Gene Reports. PII: S2452-0144(19)30094-9.

  • Roshdy F. M.Farag, E El-Ahwany, O. Mahmoud, A. Mousa, R. Ibrahim, F.Essawy (2019). Long Non-Coding RNA H19 As Potential Biomarker for HCV Genotype 4 Induced Hepatocellular Carcinoma Patients. Azhar Journal of Pharmaceutical Science, Vol. 60, DOI: 10.21608/AJPS.2019.70241.

  • Saadi G, Khaled I, Mohammed AA, Ismail W, El-Ansary M and Ramadan R (2019). Up regulation of CD133 and CD44 as Markers of Resident Renal Progenitor cells following BM-MSCs transfusion for renal regeneration. Cancer Science & Research, 2: 1-4.

  • Zayed SA, NM Zahran, MM Khorshied, AO Abdel‐Aziz, O Mahmoud (2019).Genetic variations in death receptor domain 4 gene and the susceptibility to hepatitis C related hepatocellular carcinoma. Journal of medical virology 91 (8), 1537-1544.

  • Khafagy HF, Samhan YM, Ebied RS, Abd El-Ghany SS, Sabry OHussein NA, Badawy RI, Talaat NN, Helmy AH and El-Fandy GG (2018). Perioperative effects of desflurane versus propofol on hemostasis guided by thromboelastometry in splenectomy with liver cirrhosis. Egyptian Journal of Anaesthesia,35 (1) 1–6.

  • Madkour BA, GadA , Hamdy MS, ZahranN, Aboul-Ezz M  (2018): Interleukin -6-572 promotoer gene polymorphism and its association with chronic hepatitis C-induced hepatocellular carcinoma:an Egyptian study. Comparative Clinical Pathology,27:161-165.

  • Madkour BA, Mahmoud OM, Sabry OM, Aboul Ezz MA. Association between epidermal growth factor gene functional polymorphism and hepato cellular carcinoma in a cohort of Egyptien patients. Presented as poster in United Confrenece of Gastroenterology and infectious Diseases (UCHID).Towards the unity and filling the gaps”.24-26 November 2018 Cairo Egypt.

  • Salah M, Madkour BA, Fouad R, Essawy F, Tawfeek K, El-baz TM and Abdel Aziz AM (2018): The polymorphism of tumor necrosis factor alpha and hepatitis c virus related hcc.Int J Adv Res.,6(3): 1140-1146.

  • Zahran N, Sabry O, Raafat M (2018). Evaluating the effects of recombinant human-granulocyte macrophage colony stimulating factor (rh-GMCSF) on neutrophil expression of tumor protein 53 (P53) and B cell chronic lymphocytic leukemia/lymphoma 2 (BCL-2) proteins in chronic kidney disease and hemodialysis patients: A Pilot Study. Journal of  Medical  Science,; (1): 17-23

  • El-Bassiouni NE, AminNA, El AmirAM, Farid AA, Madkour ME, Raafat I. Atta (2017). Down regulation of classical monocytes subset in patients with HCV relatedLiver fibrosis. J Egyp Soc Parasitol.,47: 207-2010.

  • Hassan RM, Khorshed SE, Atta R, Sabry O, Abdel Rahim A, Mashaal  AR and Abdel Moneam M (2017). Lens culinaris aglutinin reactive 3 alpha fetoprotein as a diagnostic serum marker for hepatocellular carcinoma with normal alpha fetoprotein level. Advances in Biological Research,11 (4): 212-220, 2017.

  • Hussein NA, HH El-Sayed, OM Mahmoud, MM Nosseir, OM Sabry, ME Attia, FM Essawy (2017). Toll-like receptors and nuclear factor kappa-B as a cross bridge between inflammation and carcinogenesis in chronic viral hepatitis. Der Pharma Chemica,9(14): 53-60.

  • Khaled IA, Hassan M, Ghanam ME, Hammam O, Sabry O and Wahdan M (2017). Autologous hematopoietic stem cell transplantation in sub-terminal liver disease patients.  International Journal for Advanced Research,6 (3): 756-769.

  • Zakria D, Abdel Rahim A, Safwat W, Abdel Aziz A, Guda M, Sabry O, Mansour KA and Yehia Z (2017). Role of Rifaximin and Zinc in Secondary Prevention of Spontaneous Bacterial Peritonitis in Cirrhotic Patients. World Journal of Medical Science,13 (4): 255-261.

  • Essawy F, Mahmoud OM, Zahran MY, William I, Madkour ME, Sabry O, Atta R, Anas A (2016). Endothelium Derived Microparticles: A Reliable Biomarker for Detection of Portal Hypertension in Chronic Hepatitis C Patients. Advances in Environmental Biology,10 (7): 53-61.

  • Hassanein A, El-Sayed H, Zahran M, Sabry O, Wahdan M and Hamdy D (2016). In Vitro Quality Evaluation of 8 Days Storage of Random Donor Platelets Using Platelet Additive Solution.  Advances in Environmental Biology,10 (6): 131-138.

  • Nour H, Zahran N, Abd Elhamid S, Zahran MY, Raafat M and Saeed A (2016). The Role of BCL-2 and BAK Genes In Chronic Kidney Disease and Haemodialysis Patients. Journal of Glycomics and Metabolism,1(1).

  • Zahran M, Nasr FM, Metwaly AA, El- Sheikh N, Khalil NSA, Harba T(2016).The Role of Hemostatic Factors in Atherosclerosis in Patients with Chronic Renal Disease. Electronic Physician,7(5):1270- 1276.

  • Zahran N, Khaled IA, Hammam O, El-Mansy M, Hassanein A, Sabry O and Ramadan A (2016). Study of Potential Differentiation of Wharton’s Jelly-Derived Umbilical Cord-Mesenchymal Stem Cells into Renal Tissues. Middle-East Journal of Scientific Research,24 (11): 3380-3387.

  • El-Bassiouni NE, RM Benyamin, SH Risk, Zahran MYMahmoud OM, Ibrahim R, El Bassiouny A (2015). The Analysis of Mutual Relationship between Hemostasis and Inflammation Processes in Patients with Chronic Liver Diseases. Blood, 126:1015.    

  • El Gendy H, Madkour BA, Abdelaty S, Essawy F, Khattab D, Hammam O, El Kholy A, Nour HH (2014): Galectin 3 for the diagnosis of bladder cancer.  Arab Journal of Urology,12: 178 -181.

  • El-Sayed H, Hassanein A, Fawzy R, Zahran MY, Mahmoud OM, Yacoub E, and Moustafa A (2014). Impact of Different Preparation Methods on the In Vitro Quality of 8 Days Storage Platelet Concentrates. Life Science Journal,11(1): 50-57.

  • KhafagyHF, HusseinNA, MadkourME, MahmoudOM, MansourAZ, YussifMF, AbbasAA, Samhan YM (2014). Perioperative effects of anesthesia and surgery on inflammation-coagulation interaction.Life Science Journal, 11(12): 900-906.

  • Yousri  M, Assem M, Helaly A , El Azab G, Safwat  W, Anas A, Zahran M (2013). APRI as a predictor for sustained virological response in chronic hepatitis C patients genotype 4.   J American Science,9: 265-269.

  • Gadalla A, Al Gendy A, Hashim AM,  Amer MM, Sabry OM (2013). Effects of Immobilization Stress and Adrenomodullin on Interleukin-10 Levels in Some Rat Tissues. Bulletin of Egyptian Society Physiological Science, 33 (2): 161- 170.

  • Zahran N, Raafat M, Sayed A, William I, Mahmoud OM, Sabry O (2013). Neutrophil apoptosis: impact of granulocyte macrophage colony stimulating factor on cell survival and viability in chronic kidney disease and hemodialysis patients. Archives of Medical Science,9 (6): 984–989

  • Zahran NM, Mahmoud OM, Zahran MY, Sayed AH, Raffat M (2013). Role of GM-CSF in Normalizing the Effect of Uremic Toxins on Neutrophil Apoptosis and Function.  J American Science, 10(12s).

  • Zahran NM, Sayed AH, Bekheet IW, Mahmoud OM, Sabry OM, Raffat M (2013).  Neutrophil apoptosis: impact of granulocyte macrophage colony stimulating factor on cell survival and viability in chronic kidney disease and hemodialysis patients. Arch Med Sci.,9: 984–989.

  • Zahran NM, Bekheet IW, Sayed AHZahran MY, Sabry OM, Rafaat M (2012). Granulocyte Macrophage Colony Stimulating Factor Mediated Modulation of Polymorphonuclear Leucocyte Dysfunction in Chronic Kidney Disease and Hemodialysis Patients. J Am Sci.,8: 6-10.

  • Zahran N, William I, Sayed A, Zahran MY, Sabry O and Raafat M (2012). Granulocyte Macrophage Colony Stimulating Factor Mediated Modulation of Polymorphonuclear Leukocyte Dysfunction in Chronic Kidney Disease and Hemodialysis Patients. Journal of American Science,8 (6): 731-737.

  • Raafat M, Metwaly A, Abdel-Khalik A, Abo-Zikri N, Madkour M, Hussein N (2012).  Inflammatory and nutritional biomarkers: Role as non-traditional risk factors for cardiovascular morbidity in patients with chronic kidney disease. J Life Sci,9: 1109-1116.

  • Madkour BA, Bekheet IW, Mahgoub IS, Samy MM, Roshdy M, Essawy FM (2012). Platelet and neutrophil cross-talk-mediating cancer growth and metastasis in patients with urinary bladder cancer.  J Arab Society for Medical Research, 7: 1–9.

  • Zahran NM, Sayed AH, Bekheet IW, Mahmoud OM, Sabry OM, Manar R (2011).  Neutrophil apoptosis: Impact of GM-CSF on cell survival and viability in chronic kidney disease and hemodialysis patients. Arch Med Sci,6: 432-438.

  • El Bassiouny AE, NosseirMM, ZoheiryMK, NA Amin, Abdel-HadiAM, Ibrahim IM, ZadaS, Saad El-DinA, El–Bassiouni NE (2010):   Differential expression of Cell cycle regulators in HCV infection and related hepatocellular Carcinoma.  World Journal of Hepatology,2 (1): 32-41

  • Madkour BA, Bekheet IW, Khaled IA, El Baz A (2009). Platelet activation.A link between angiogenesis and haemostasis in bladder cancer patients. J Med research and Sciences, 31 March.

  • Zahran NM, Sayed AH, Abdel-Latif R, Hassanein S, Ibrahim R, Essawy FM (2009). Soluble Intercellular Adhesion Molecule-1 and Lymphocyte Function; Associated Antigen-1 in Viral Related Chronic Liver Diseases. New Egypt J Med,40: 333-41.

  • Madkour ME, Hussein N, Bekheet IW, Waked E, Rafat M, Essawy FM (2008). The relative roles of stimulators and inhibitors of erythropoiesis in development of renal anemia. African J Nephrol,12: 7-14.

  • Toiema SM, Bekheet I, Zahran YMadkour M, Rafaat M (2008). Possible role of G-CSF on neutrophil activation, endothelial dysfunction and haemostatic changes in chronic renal failure patients.haemostasis in bladder cancer patients.Egypt J Med,3: 142-148.  

Prof. Sawsan A. Omran: The State Award in the field of Medicine, 1984.
Prof. Sawsan A. Omran: The State Award in the field of Tropical Medicine and Haematology, 1994.
Prof. Nora E. El-Bassiouni: The Islamic Development Bank Merit Scholarship Programme Award, 1996.
Prof. Nora E. El-Bassiouni: The annual Professor Sawsan Omran’s Award for the best research article, 2009.
Prof. Nariman A. Zahran: The annual Professor Sawsan Omran’s award for the best research article, 2002.
Ass. Prof. Azza S. Hassanien: The annual Professor Sawsan Omran’s award for the best research article, 2011.